Recombinant
RabMAb

Recombinant Anti-alpha 1 Spectrin antibody [EPR9299] (ab154811)

Overview

  • Product name

    Anti-alpha 1 Spectrin antibody [EPR9299]
    See all alpha 1 Spectrin primary antibodies
  • Description

    Rabbit monoclonal [EPR9299] to alpha 1 Spectrin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human alpha 1 Spectrin. The exact sequence is proprietary.

  • Positive control

    • K562 and TF-1 cell lysates; Human lung and stomach tissues
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 280 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
    • Involvement in disease

      Defects in SPTA1 are the cause of elliptocytosis type 2 (EL2) [MIM:130600]. EL2 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape.
      Defects in SPTA1 are a cause of hereditary pyropoikilocytosis (HPP) [MIM:266140]. HPP is an autosomal recessive disorder characterized by hemolytic anemia, microspherocytosis, poikilocytosis, and an unusual thermal sensitivity of red cells.
      Defects in SPTA1 are the cause of spherocytosis type 3 (SPH3) [MIM:270970]; also known as hereditary spherocytosis type 3 (HS3). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH3 is characterized by severe hemolytic anemia. Inheritance is autosomal recessive.
    • Sequence similarities

      Belongs to the spectrin family.
      Contains 3 EF-hand domains.
      Contains 1 SH3 domain.
      Contains 21 spectrin repeats.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cytoplasm > cell cortex.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alpha I spectrin antibody
      • EL 2 antibody
      • EL2 antibody
      • Elliptocytosis 2 antibody
      • Elliptocytosis2 antibody
      • Erythrocyte alpha spectrin antibody
      • erythrocyte antibody
      • Erythroid alpha spectrin antibody
      • Erythroid alpha-spectrin antibody
      • Erythroid spectrin alpha antibody
      • HPP antibody
      • HS3 antibody
      • Spectrin alpha chain antibody
      • Spectrin alpha chain erythrocyte antibody
      • Spectrin alpha erythrocytic 1 antibody
      • SPH3 antibody
      • SPTA 1 antibody
      • SPTA antibody
      • SPTA1 antibody
      • SPTA1_HUMAN antibody
      see all

    Images

    • All lanes : Anti-alpha 1 Spectrin antibody [EPR9299] (ab154811) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : TF-1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 280 kDa

    • Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling alpha 1 Spectrin with ab154811 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling alpha 1 Spectrin with ab154811 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin embedded Human normal liver, erythrocytes tissue using ab154811 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human placenta, erythrocytes tissue using ab154811 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal spleen, erythrocytes tissue using ab154811 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal uterus, erythrocytes tissue using ab154811 showing +ve staining.

    References

    ab154811 has not yet been referenced specifically in any publications.

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