Overview

  • Product name

    Anti-alpha 1 Spectrin antibody [EPR9300]
    See all alpha 1 Spectrin primary antibodies
  • Description

    Rabbit monoclonal [EPR9300] to alpha 1 Spectrin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human alpha 1 Spectrin aa 2300-2400. The exact sequence is proprietary.

  • Positive control

    • K562, Human fetal heart and fetal liver lysates; K562 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab139403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 280 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
    • Involvement in disease

      Defects in SPTA1 are the cause of elliptocytosis type 2 (EL2) [MIM:130600]. EL2 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape.
      Defects in SPTA1 are a cause of hereditary pyropoikilocytosis (HPP) [MIM:266140]. HPP is an autosomal recessive disorder characterized by hemolytic anemia, microspherocytosis, poikilocytosis, and an unusual thermal sensitivity of red cells.
      Defects in SPTA1 are the cause of spherocytosis type 3 (SPH3) [MIM:270970]; also known as hereditary spherocytosis type 3 (HS3). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH3 is characterized by severe hemolytic anemia. Inheritance is autosomal recessive.
    • Sequence similarities

      Belongs to the spectrin family.
      Contains 3 EF-hand domains.
      Contains 1 SH3 domain.
      Contains 21 spectrin repeats.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cytoplasm > cell cortex.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alpha I spectrin antibody
      • EL 2 antibody
      • EL2 antibody
      • Elliptocytosis 2 antibody
      • Elliptocytosis2 antibody
      • Erythrocyte alpha spectrin antibody
      • erythrocyte antibody
      • Erythroid alpha spectrin antibody
      • Erythroid alpha-spectrin antibody
      • Erythroid spectrin alpha antibody
      • HPP antibody
      • HS3 antibody
      • Spectrin alpha chain antibody
      • Spectrin alpha chain erythrocyte antibody
      • Spectrin alpha erythrocytic 1 antibody
      • SPH3 antibody
      • SPTA 1 antibody
      • SPTA antibody
      • SPTA1 antibody
      • SPTA1_HUMAN antibody
      see all

    Images

    • All lanes : Anti-alpha 1 Spectrin antibody [EPR9300] (ab139403) at 1/1000 dilution

      Lane 1 : K562 lysate
      Lane 2 : Human fetal heart lysate
      Lane 3 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 280 kDa

    • Flow Cytometric analysis of permeabilized K562 cells labelling alpha 1 Spectrin with ab139403 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    • Vorselen D  et al. The fluid membrane determines mechanics of erythrocyte extracellular vesicles and is softened in hereditary spherocytosis. Nat Commun 9:4960 (2018). Read more (PubMed: 30470753) »
    See 1 Publication for this product

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