Recombinant
RabMAb

Recombinant Anti-alpha 1 Spectrin antibody [EPR9300] - BSA and Azide free (ab248875)

Overview

  • Product name

    Anti-alpha 1 Spectrin antibody [EPR9300] - BSA and Azide free
    See all alpha 1 Spectrin primary antibodies
  • Description

    Rabbit monoclonal [EPR9300] to alpha 1 Spectrin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human alpha 1 Spectrin aa 2300-2400. The exact sequence is proprietary.

  • General notes

    Ab248875 is the carrier-free version of ab139403. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab248875 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab248875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 280 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
    • Involvement in disease

      Defects in SPTA1 are the cause of elliptocytosis type 2 (EL2) [MIM:130600]. EL2 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape.
      Defects in SPTA1 are a cause of hereditary pyropoikilocytosis (HPP) [MIM:266140]. HPP is an autosomal recessive disorder characterized by hemolytic anemia, microspherocytosis, poikilocytosis, and an unusual thermal sensitivity of red cells.
      Defects in SPTA1 are the cause of spherocytosis type 3 (SPH3) [MIM:270970]; also known as hereditary spherocytosis type 3 (HS3). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH3 is characterized by severe hemolytic anemia. Inheritance is autosomal recessive.
    • Sequence similarities

      Belongs to the spectrin family.
      Contains 3 EF-hand domains.
      Contains 1 SH3 domain.
      Contains 21 spectrin repeats.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cytoplasm > cell cortex.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alpha I spectrin antibody
      • EL 2 antibody
      • EL2 antibody
      • Elliptocytosis 2 antibody
      • Elliptocytosis2 antibody
      • Erythrocyte alpha spectrin antibody
      • erythrocyte antibody
      • Erythroid alpha spectrin antibody
      • Erythroid alpha-spectrin antibody
      • Erythroid spectrin alpha antibody
      • HPP antibody
      • HS3 antibody
      • Spectrin alpha chain antibody
      • Spectrin alpha chain erythrocyte antibody
      • Spectrin alpha erythrocytic 1 antibody
      • SPH3 antibody
      • SPTA 1 antibody
      • SPTA antibody
      • SPTA1 antibody
      • SPTA1_HUMAN antibody
      see all

    References

    ab248875 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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