Recombinant
RabMAb

Recombinant Anti-alpha 1,2 Mannosidase IA/MAN1A1 antibody [EPR9957(B)] (ab140613)

Overview

  • Product name

    Anti-alpha 1,2 Mannosidase IA/MAN1A1 antibody [EPR9957(B)]
    See all alpha 1,2 Mannosidase IA/MAN1A1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9957(B)] to alpha 1,2 Mannosidase IA/MAN1A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human alpha 1,2 Mannosidase IA/MAN1A1 aa 600-700 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HepG2 and 293T cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as alpha 1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab140613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2).
    • Pathway

      Protein modification; protein glycosylation.
    • Sequence similarities

      Belongs to the glycosyl hydrolase 47 family.
    • Cellular localization

      Golgi apparatus membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2-alpha-mannosidase IA antibody
      • 2-mannosidase IA antibody
      • Alpha 1,2 mannosidase IA antibody
      • Alpha-1 antibody
      • EC 3.2.1.113 antibody
      • HUMM3 antibody
      • HUMM9 antibody
      • MA1A1_HUMAN antibody
      • Man(9) alpha mannosidase antibody
      • Man(9)-alpha-mannosidase antibody
      • MAN1A1 antibody
      • MAN9 antibody
      • Man9 mannosidase antibody
      • Man9-mannosidase antibody
      • Mannosidase alpha class 1A member 1 antibody
      • Mannosyl-oligosaccharide 1 antibody
      • Mannosyl-oligosaccharide 1,2 alpha mannosidase IA antibody
      • OTTHUMP00000017107 antibody
      • Processing alpha 1,2 mannosidase IA antibody
      • Processing alpha-1 antibody
      see all

    Images

    • All lanes : Anti-alpha 1,2 Mannosidase IA/MAN1A1 antibody [EPR9957(B)] (ab140613) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 73 kDa
      Observed band size: 73 kDa

    References

    This product has been referenced in:

    • Casey CA  et al. Study of Ethanol-Induced Golgi Disorganization Reveals the Potential Mechanism of Alcohol-Impaired N-Glycosylation. Alcohol Clin Exp Res 40:2573-2590 (2016). Read more (PubMed: 27748959) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mice hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    Mice hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde

    Dr. Armen Petrosyan

    Verified customer

    Submitted Jul 22 2019

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Rat hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    Rat hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Mar 18 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HepG2 cells)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    HepG2 cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Mar 18 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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