Recombinant Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)
![Flow Cytometry (Intracellular) - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)](https://www.abcam.com/ps/products/137/ab137123/Images/ab137123-204522-anti-alpha-1a-adrenergic-receptor-antibody-epr9691b-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9691(B)] to alpha 1a Adrenergic Receptor/ADRA1A
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)]
See all alpha 1a Adrenergic Receptor/ADRA1A primary antibodies -
Description
Rabbit monoclonal [EPR9691(B)] to alpha 1a Adrenergic Receptor/ADRA1A -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human alpha 1a Adrenergic Receptor/ADRA1A aa 200-300. The exact sequence is proprietary.
Database link: P35348 -
Positive control
- PC-3, HepG2, and Raji cell lysates, HepG2 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9691(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 60 kDa).
|
| ICC/IF |
1/250 - 1/500.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 60 kDa). |
|
ICC/IF
1/250 - 1/500. |
Target
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Function
This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. -
Tissue specificity
Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethra and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1A sub-subfamily. -
Post-translational
modificationsCarboxyl-terminal Ser or Thr residues may be phosphorylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 148 Human
- Entrez Gene: 11549 Mouse
- Entrez Gene: 29412 Rat
- Omim: 104221 Human
- SwissProt: P35348 Human
- SwissProt: P97718 Mouse
- SwissProt: P43140 Rat
- Unigene: 709175 Human
see all -
Alternative names
- ADA1A_HUMAN antibody
- ADRA1A antibody
- Adra1c antibody
see all
Images
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Flow Cytometry (Intracellular) - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)
Overlay histogram showing HepG2 cells stained with ab137123 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137123, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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![Western blot - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)](https://www.abcam.com/ps/products/137/ab137123/Images/ab137123-151747-anti-alpha-1a-adrenergic-receptor-antibody-epr9691b-western-blot.jpg)
Western blot - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)All lanes : Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123) at 1/1000 dilution
Lane 1 : PC-3 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)](https://www.abcam.com/ps/products/137/ab137123/Images/ab137123-151746-anti-alpha-1a-adrenergic-receptor-antibody-epr9691b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)Immunofluorescent analysis of HepG2 cells labelling alpha 1a Adrenergic Receptor/ADRA1A with ab137123 at 1/250 dilution.
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![Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)](https://www.abcam.com/ps/products/137/ab137123/Images/ab137123-4-benefits-of-recombinant-antibodies.png)
Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)
Protocols
Datasheets and documents
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Datasheet download
References (16)
ab137123 has been referenced in 16 publications.
- D'Adamo P et al. Inhibiting glycolysis rescues memory impairment in an intellectual disability Gdi1-null mouse. Metabolism 116:154463 (2021). PubMed: 33309713
- Lee JY et al. Expression of bladder a1-adrenoceptor subtype after relief of partial bladder outlet obstruction in a rat model. Investig Clin Urol 61:297-303 (2020). PubMed: 32377606
- Florent R et al. Bim, Puma and Noxa upregulation by Naftopidil sensitizes ovarian cancer to the BH3-mimetic ABT-737 and the MEK inhibitor Trametinib. Cell Death Dis 11:380 (2020). PubMed: 32424251
- Liu X et al. Distribution and relative expression of vasoactive receptors on arteries. Sci Rep 10:15383 (2020). PubMed: 32958803
- Christoffersson G et al. Interference with pancreatic sympathetic signaling halts the onset of diabetes in mice. Sci Adv 6:N/A (2020). PubMed: 33052874
