RecombinantRabMAb

Recombinant Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)

Reviews (1)Q&A (2)References (16)
Flow Cytometry (Intracellular) - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)
  • Western blot - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)
  • Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9691(B)] to alpha 1a Adrenergic Receptor/ADRA1A
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free PE

Overview

  • Product name

    Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)]
    See all alpha 1a Adrenergic Receptor/ADRA1A primary antibodies

  • Description

    Rabbit monoclonal [EPR9691(B)] to alpha 1a Adrenergic Receptor/ADRA1A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human alpha 1a Adrenergic Receptor/ADRA1A aa 200-300. The exact sequence is proprietary.
    Database link: P35348

  • Positive control

    • PC-3, HepG2, and Raji cell lysates, HepG2 cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 60 kDa).
ICC/IF
1/250 - 1/500.
Notes
Flow Cyt (Intra)
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 60 kDa).
ICC/IF
1/250 - 1/500.

Target

  • Function

    This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins.

  • Tissue specificity

    Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethra and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1A sub-subfamily.

  • Post-translational
    modifications

    Carboxyl-terminal Ser or Thr residues may be phosphorylated.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ADA1A_HUMAN antibody
    • ADRA1A antibody
    • Adra1c antibody
    • ADRA1L1 antibody
    • Adrenergic alpha1A receptor antibody
    • Adrenergic alpha1C receptor antibody
    • Adrenergic receptor alpha 1a antibody
    • Adrenergic receptor alpha 1c antibody
    • Adrenergic, alpha 1A , receptor variant 1 antibody
    • Adrenergic, alpha 1A , receptor variant 11 antibody
    • Adrenergic, alpha 1A , receptor variant 3 antibody
    • Adrenergic, alpha 1A , receptor variant 5 antibody
    • Adrenergic, alpha 1A , receptor variant 8 antibody
    • Adrenoceptor alpha 1A antibody
    • Alpha 1A adrenoreceptor antibody
    • Alpha 1C adrenergic receptor antibody
    • Alpha adrenergic receptor 1c antibody
    • Alpha-1A adrenergic receptor antibody
    • Alpha-1A adrenoceptor antibody
    • Alpha-1A adrenoreceptor antibody
    • Alpha-1C adrenergic receptor antibody
    • Alpha-adrenergic receptor 1c antibody
    • Alpha1A adrenergic receptor antibody
    • ALPHA1AAR antibody
    • G protein coupled receptor antibody
    see all

Images

  • Flow Cytometry (Intracellular) - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)
    Flow Cytometry (Intracellular) - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)

    Overlay histogram showing HepG2 cells stained with ab137123 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137123, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

     

  • Western blot - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)
    Western blot - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)
    All lanes : Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123) at 1/1000 dilution

    Lane 1 : PC-3 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 60 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)

    Immunofluorescent analysis of HepG2 cells labelling alpha 1a Adrenergic Receptor/ADRA1A with ab137123 at 1/250 dilution.

  • Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)
    Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)

References (16)

Publishing research using ab137123? Please let us know so that we can cite the reference in this datasheet.

ab137123 has been referenced in 16 publications.

  • D'Adamo P  et al. Inhibiting glycolysis rescues memory impairment in an intellectual disability Gdi1-null mouse. Metabolism 116:154463 (2021). PubMed: 33309713
  • Lee JY  et al. Expression of bladder a1-adrenoceptor subtype after relief of partial bladder outlet obstruction in a rat model. Investig Clin Urol 61:297-303 (2020). PubMed: 32377606
  • Florent R  et al. Bim, Puma and Noxa upregulation by Naftopidil sensitizes ovarian cancer to the BH3-mimetic ABT-737 and the MEK inhibitor Trametinib. Cell Death Dis 11:380 (2020). PubMed: 32424251
  • Liu X  et al. Distribution and relative expression of vasoactive receptors on arteries. Sci Rep 10:15383 (2020). PubMed: 32958803
  • Christoffersson G  et al. Interference with pancreatic sympathetic signaling halts the onset of diabetes in mice. Sci Adv 6:N/A (2020). PubMed: 33052874
View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat brain,Mouse brain,SKNSH,HepaRG,HepG2,293T)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
40 µg
Specification
Rat brain,Mouse brain,SKNSH,HepaRG,HepG2,293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Dr. Romain Parent

Verified customer

Submitted Aug 04 2021

Question

does this antibody recognize all isoforms?

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Answer

Thank you for contacting us. Although the specificity has not been tested, the immunogen is taken from the portion of the protein which is the same in all isofrms, so this antibosy should act as a pan alpha 1 adrenergic receptor antibody.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

I am having difficulty identifying antibodies and I was hoping that Abcam might offer what I am looking for.



I am looking for primary antibodies to use for flow cytometry and reactivity is human.



I need 7 antibodies total:

Adrenergic receptor alpha-1A (I think I may have identified this one as ab137123)

Dopamine receptor D1

Adrenergic Receptor alpha-2A

Serotonin receptor 4 (5-HT4)

Serotonin receptor 2C (5-HT2C)

Dopamine Receptor D3

Opioid receptor mu1



Could someone please help me find what I am looking for?

Read More
Answer

Thank you for contacting Abcam.

We have antibodies for Adrenergic receptor alpha-1A and Dopamine receptor D1 in our catalogue that match your requirements and the links to those are below:

Adrenergic receptor alpha-1A
https://www.abcam.com/alpha-1-Adrenergic-Receptor-antibody-EPR9691B-ab137123.html

Dopamine receptor D1
https://www.abcam.com/Dopamine-Receptor-D1-antibody-EP1560Y-ab81296.html

For the rest of the targets that you are searching for, we do not have any that work in both human and flow cytometry and so I have placed links to the best ones we have in the catalogue that are likely to work for you. As we cannot guarantee that they will work, for the targets below you could take advantage of our AbTrial program (https://www.abcam.com/abtrial):

Adrenergic Receptor alpha-2A
We do not have any that match your requirements, but below is the link to one that may work for you in Flow Cytometry:
https://www.abcam.com/5HT4-Receptor-antibody-ab60359.html

Serotonin receptor 4 (5-HT4)
We do not have any that match your requirements, but below is the link to one that may work for you in Flow Cytometry:
https://www.abcam.com/5HT4-Receptor-antibody-ab60359.html

Serotonin receptor 2C (5-HT2C)
We do not have any that match your requirements, but below is the link to one that may work for you in Flow Cytometry:
https://www.abcam.com/5HT2C-Receptor-antibody-ab32887.html

Dopamine Receptor D3
We do not have any that match your requirements, but below is the link to one that may work for you in Flow Cytometry:
https://www.abcam.com/Dopamine-Receptor-D3-antibody-ab42114.html

Opioid receptor mu1
We do not have any that match your requirements, but below is the link to one that may work for you in Flow Cytometry:
https://www.abcam.com/Mu-Opioid-Receptor-antibody-UMB3-ab134054.html

If there is anything else I can help you with, or if you would like to take advantage of the AbTrial program, then please let me know.

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