Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9691(B)] to alpha 1a Adrenergic Receptor/ADRA1A
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)]
See all alpha 1a Adrenergic Receptor/ADRA1A primary antibodies
DescriptionRabbit monoclonal [EPR9691(B)] to alpha 1a Adrenergic Receptor/ADRA1A
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human alpha 1a Adrenergic Receptor/ADRA1A aa 200-300. The exact sequence is proprietary.
Database link: P35348
- PC-3, HepG2, and Raji cell lysates, HepG2 cells
This product was previously labelled as alpha 1a Adrenergic Receptor
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab137123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 60 kDa).|
|ICC/IF||1/250 - 1/500.|
|Flow Cyt||1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThis alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins.
Tissue specificityExpressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethra and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1A sub-subfamily.
modificationsCarboxyl-terminal Ser or Thr residues may be phosphorylated.
Cellular localizationCell membrane.
- Information by UniProt
- ADA1A_HUMAN antibody
- ADRA1A antibody
- Adra1c antibody
Overlay histogram showing HepG2 cells stained with ab137123 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137123, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
All lanes : Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123) at 1/1000 dilution
Lane 1 : PC-3 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Immunofluorescent analysis of HepG2 cells labelling alpha 1a Adrenergic Receptor/ADRA1A with ab137123 at 1/250 dilution.
ab137123 has been referenced in 10 publications.
- Qin X et al. Discovery of Environment-Sensitive Fluorescent Agonists for a1-Adrenergic Receptors. Anal Chem 91:12173-12180 (2019). PubMed: 31321979
- Tang JM et al. Response Gene to Complement 32 Maintains Blood Pressure Homeostasis by Regulating a-Adrenergic Receptor Expression. Circ Res 123:1080-1090 (2018). PubMed: 30355157
- Tyurin-Kuzmin PA et al. Noradrenaline Sensitivity Is Severely Impaired in Immortalized Adipose-Derived Mesenchymal Stem Cell Line. Int J Mol Sci 19:N/A (2018). PubMed: 30469522
- Kawai H et al. Time of Administration of Acute or Chronic Doses of Imipramine Affects its Antidepressant Action in Rats. J Circadian Rhythms 16:5 (2018). PubMed: 30210565
- Tyurin-Kuzmin PA et al. Flow cytometry analysis of adrenoceptors expression in human adipose-derived mesenchymal stem/stromal cells. Sci Data 5:180196 (2018). PubMed: 30277480
- Rice FL et al. Anatomy and immunochemical characterization of the non-arterial peptidergic diffuse dural innervation of the rat and Rhesus monkey: Implications for functional regulation and treatment in migraine. Cephalalgia 37:1350-1372 (2017). PubMed: 27852962
- Albee LJ et al. a1-Adrenergic Receptors Function Within Hetero-Oligomeric Complexes With Atypical Chemokine Receptor 3 and Chemokine (C-X-C motif) Receptor 4 in Vascular Smooth Muscle Cells. J Am Heart Assoc 6:N/A (2017). PubMed: 28862946
- Evans AE et al. New Insights into Mechanisms and Functions of Chemokine (C-X-C Motif) Receptor 4 Heteromerization in Vascular Smooth Muscle. Int J Mol Sci 17:N/A (2016). Flow Cyt . PubMed: 27331810
- Tyurin-Kuzmin PA et al. Activation of ß-adrenergic receptors is required for elevated a1A-adrenoreceptors expression and signaling in mesenchymal stromal cells. Sci Rep 6:32835 (2016). Flow Cyt . PubMed: 27596381
- Tripathi A et al. Heteromerization of chemokine (C-X-C motif) receptor 4 with a1A/B-adrenergic receptors controls a1-adrenergic receptor function. Proc Natl Acad Sci U S A 112:E1659-68 (2015). PubMed: 25775528