Recombinant
RabMAb

Recombinant Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (Alexa Fluor® 647) (ab225190)

Overview

  • Product name

    Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (Alexa Fluor® 647)
    See all alpha 1a Adrenergic Receptor/ADRA1A primary antibodies
  • Description

    Rabbit monoclonal [EPR9691(B)] to alpha 1a Adrenergic Receptor/ADRA1A (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human alpha 1a Adrenergic Receptor/ADRA1A aa 200-300. The exact sequence is proprietary.

  • Positive control

    • ICC/IF: Raji cells. Flow Cyt: Raji cells
  • General notes

     This product was previously labelled as alpha 1a Adrenergic Receptor

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9691(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab225190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/5000.

This product gave a positive signal in Raji cells fixed with 4% formaldehyde (10 min)

Flow Cyt 1/5000.

Target

  • Function

    This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins.
  • Tissue specificity

    Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethra and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1A sub-subfamily.
  • Post-translational
    modifications

    Carboxyl-terminal Ser or Thr residues may be phosphorylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADA1A_HUMAN antibody
    • ADRA1A antibody
    • Adra1c antibody
    • ADRA1L1 antibody
    • Adrenergic alpha1A receptor antibody
    • Adrenergic alpha1C receptor antibody
    • Adrenergic receptor alpha 1a antibody
    • Adrenergic receptor alpha 1c antibody
    • Adrenergic, alpha 1A , receptor variant 1 antibody
    • Adrenergic, alpha 1A , receptor variant 11 antibody
    • Adrenergic, alpha 1A , receptor variant 3 antibody
    • Adrenergic, alpha 1A , receptor variant 5 antibody
    • Adrenergic, alpha 1A , receptor variant 8 antibody
    • Adrenoceptor alpha 1A antibody
    • Alpha 1A adrenoreceptor antibody
    • Alpha 1C adrenergic receptor antibody
    • Alpha adrenergic receptor 1c antibody
    • Alpha-1A adrenergic receptor antibody
    • Alpha-1A adrenoceptor antibody
    • Alpha-1A adrenoreceptor antibody
    • Alpha-1C adrenergic receptor antibody
    • Alpha-adrenergic receptor 1c antibody
    • Alpha1A adrenergic receptor antibody
    • ALPHA1AAR antibody
    • G protein coupled receptor antibody
    see all

Images

  • Overlay histogram showing Raji cells stained with ab225190 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225190, 1/5000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

  • ab225190 staining alpha 1a Adrenergic Receptor/ADRA1A in Raji cells. The cells were fixed with 4% formaldehyde (10 min) and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab225190 at 1/5000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab225190 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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