Recombinant
RabMAb

Recombinant Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] - BSA and Azide free (ab238923)

Overview

  • Product name

    Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] - BSA and Azide free
    See all alpha 1a Adrenergic Receptor/ADRA1A primary antibodies
  • Description

    Rabbit monoclonal [EPR9691(B)] to alpha 1a Adrenergic Receptor/ADRA1A - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human alpha 1a Adrenergic Receptor/ADRA1A aa 200-300. The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HepG2 cells.
  • General notes

    ab238923 is the carrier-free version of ab137123 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab238923 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as alpha 1a Adrenergic Receptor

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9691(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab238923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 60 kDa).

Target

  • Function

    This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins.
  • Tissue specificity

    Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethra and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1A sub-subfamily.
  • Post-translational
    modifications

    Carboxyl-terminal Ser or Thr residues may be phosphorylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADA1A_HUMAN antibody
    • ADRA1A antibody
    • Adra1c antibody
    • ADRA1L1 antibody
    • Adrenergic alpha1A receptor antibody
    • Adrenergic alpha1C receptor antibody
    • Adrenergic receptor alpha 1a antibody
    • Adrenergic receptor alpha 1c antibody
    • Adrenergic, alpha 1A , receptor variant 1 antibody
    • Adrenergic, alpha 1A , receptor variant 11 antibody
    • Adrenergic, alpha 1A , receptor variant 3 antibody
    • Adrenergic, alpha 1A , receptor variant 5 antibody
    • Adrenergic, alpha 1A , receptor variant 8 antibody
    • Adrenoceptor alpha 1A antibody
    • Alpha 1A adrenoreceptor antibody
    • Alpha 1C adrenergic receptor antibody
    • Alpha adrenergic receptor 1c antibody
    • Alpha-1A adrenergic receptor antibody
    • Alpha-1A adrenoceptor antibody
    • Alpha-1A adrenoreceptor antibody
    • Alpha-1C adrenergic receptor antibody
    • Alpha-adrenergic receptor 1c antibody
    • Alpha1A adrenergic receptor antibody
    • ALPHA1AAR antibody
    • G protein coupled receptor antibody
    see all

Images

  • Overlay histogram showing HepG2 cells stained with ab137123 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137123, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137123).

  • Immunofluorescent analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labelling alpha 1a Adrenergic Receptor/ADRA1A with ab137123 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137123).

References

ab238923 has not yet been referenced specifically in any publications.

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