Recombinant
RabMAb

Recombinant Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (Phycoerythrin) (ab225191)

Overview

  • Product name

    Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (Phycoerythrin)
    See all alpha 1a Adrenergic Receptor/ADRA1A primary antibodies
  • Description

    Rabbit monoclonal [EPR9691(B)] to alpha 1a Adrenergic Receptor/ADRA1A (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human alpha 1a Adrenergic Receptor/ADRA1A aa 200-300. The exact sequence is proprietary.

  • Positive control

    • Flow Cyt: Raji cells
  • General notes

     This product was previously labelled as alpha 1a Adrenergic Receptor

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9691(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab225191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1000.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins.
  • Tissue specificity

    Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethra and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1A sub-subfamily.
  • Post-translational
    modifications

    Carboxyl-terminal Ser or Thr residues may be phosphorylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADA1A_HUMAN antibody
    • ADRA1A antibody
    • Adra1c antibody
    • ADRA1L1 antibody
    • Adrenergic alpha1A receptor antibody
    • Adrenergic alpha1C receptor antibody
    • Adrenergic receptor alpha 1a antibody
    • Adrenergic receptor alpha 1c antibody
    • Adrenergic, alpha 1A , receptor variant 1 antibody
    • Adrenergic, alpha 1A , receptor variant 11 antibody
    • Adrenergic, alpha 1A , receptor variant 3 antibody
    • Adrenergic, alpha 1A , receptor variant 5 antibody
    • Adrenergic, alpha 1A , receptor variant 8 antibody
    • Adrenoceptor alpha 1A antibody
    • Alpha 1A adrenoreceptor antibody
    • Alpha 1C adrenergic receptor antibody
    • Alpha adrenergic receptor 1c antibody
    • Alpha-1A adrenergic receptor antibody
    • Alpha-1A adrenoceptor antibody
    • Alpha-1A adrenoreceptor antibody
    • Alpha-1C adrenergic receptor antibody
    • Alpha-adrenergic receptor 1c antibody
    • Alpha1A adrenergic receptor antibody
    • ALPHA1AAR antibody
    • G protein coupled receptor antibody
    see all

Images

  • Overlay histogram showing Raji cells stained with ab225191 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225191, 1/1000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in Raji cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab225191 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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