Product nameAnti-alpha 1b Adrenergic Receptor antibody [EPR10336]
See all alpha 1b Adrenergic Receptor primary antibodies
DescriptionRabbit monoclonal [EPR10336] to alpha 1b Adrenergic Receptor
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human alpha 1b Adrenergic Receptor aa 150-250. The exact sequence is proprietary.
Database link: P35368
- Fetal brain, PC-3 and HeLa lysates, HeLa cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab169523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 57 kDa.|
|ICC/IF||1/250 - 1/500.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThis alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily.
Cellular localizationNucleus membrane. Cell membrane. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.
- Information by UniProt
- ADA1B_HUMAN antibody
- ADRA 1 antibody
- Adra 1b antibody
All lanes : Anti-alpha 1b Adrenergic Receptor antibody [EPR10336] (ab169523) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : PC-3 lysate
Lane 3 : HeLa lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 57 kDa
Immunofluorescent analysis of HeLa cells labeling alpha 1b Adrenergic Receptor with ab169523 at 1/250.
Flow Cytometrical analysis of HeLa cells with ab169523 antibody at a dilution of 1/100 (red) or a rabbit IgG negative (green).
This product has been referenced in:
- Gao X et al. Asymmetrical ligand-induced cross-regulation of chemokine (C-X-C motif) receptor 4 by a1-adrenergic receptors at the heteromeric receptor complex. Sci Rep 8:2730 (2018). Read more (PubMed: 29426850) »
- Albee LJ et al. a1-Adrenergic Receptors Function Within Hetero-Oligomeric Complexes With Atypical Chemokine Receptor 3 and Chemokine (C-X-C motif) Receptor 4 in Vascular Smooth Muscle Cells. J Am Heart Assoc 6:N/A (2017). Read more (PubMed: 28862946) »