Key features and details
- Rabbit monoclonal [EPSIS17] to Alpha 2 Antiplasmin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Alpha 2 Antiplasmin antibody [EPSIS17]
See all Alpha 2 Antiplasmin primary antibodies
DescriptionRabbit monoclonal [EPSIS17] to Alpha 2 Antiplasmin
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Alpha 2 Antiplasmin aa 50-150. The exact sequence is proprietary.
- HepG2 cell lysate, Human liver tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab150414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 55 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98°C, below boiling, and then let cool for 10-20 min.
FunctionSerine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.
Tissue specificityExpressed by the liver and secreted in plasma.
Involvement in diseaseDefects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID) [MIM:262850]. APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis.
Sequence similaritiesBelongs to the serpin family.
- Information by UniProt
- A2AP antibody
- A2AP_HUMAN antibody
- AAP antibody
Anti-Alpha 2 Antiplasmin antibody [EPSIS17] (ab150414) at 1/1000 dilution + HepG2 cell lysate at 10 µg
HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 55 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin embedded Human liver tissue labelling Alpha 2 Antiplasmin with ab150414 antibody at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab150414 has not yet been referenced specifically in any publications.