Overview

  • Product name

    Anti-Alpha 2 Antiplasmin antibody [EPSIS17]
    See all Alpha 2 Antiplasmin primary antibodies
  • Description

    Rabbit monoclonal [EPSIS17] to Alpha 2 Antiplasmin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Alpha 2 Antiplasmin aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HepG2 cell lysate, Human liver tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab150414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 55 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98°C, below boiling, and then let cool for 10-20 min.

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.
    • Tissue specificity

      Expressed by the liver and secreted in plasma.
    • Involvement in disease

      Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID) [MIM:262850]. APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis.
    • Sequence similarities

      Belongs to the serpin family.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • A2AP antibody
      • A2AP_HUMAN antibody
      • AAP antibody
      • Alpha 2 antiplasmin antibody
      • Alpha 2 antiplasmin pigment epithelium derived factor antibody
      • Alpha 2 AP antibody
      • ALPHA 2 PI antibody
      • Alpha 2 plasmin inhibitor antibody
      • Alpha 2 plasmin inhibitor deficiency antibody
      • Alpha-2-antiplasmin antibody
      • Alpha-2-AP antibody
      • Alpha-2-PI antibody
      • Alpha-2-plasmin inhibitor antibody
      • Antiplasmin deficiency antibody
      • API antibody
      • Plasmin inhibitor deficiency antibody
      • PLI antibody
      • Serine (or cysteine) peptidase inhibitor, clade F, member 2 antibody
      • Serine (Or cysteine) peptidase inhibitor, clade F, member 2, isoform CRA_c antibody
      • Serine (or cysteine) proteinase inhibitor, clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2 antibody
      • Serine (Or cysteine) proteinase inhibitor, clade F, member 2 antibody
      • Serine or cysteine peptidase inhibitor clade F member 2 antibody
      • Serpin F2 antibody
      • Serpin peptidase inhibitor clade F antibody
      • Serpin peptidase inhibitor, clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2 antibody
      • SERPINF2 antibody
      see all

    Images

    • Anti-Alpha 2 Antiplasmin antibody [EPSIS17] (ab150414) at 1/1000 dilution + HepG2 cell lysate at 10 µg

      Secondary
      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 55 kDa
      Observed band size: 65 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin embedded Human liver tissue labelling Alpha 2 Antiplasmin with ab150414 antibody at a dilution of 1/100.

    References

    ab150414 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab150414.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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