Anti-alpha 2 Macroglobulin antibody (ab181656)
Key features and details
- Goat polyclonal to alpha 2 Macroglobulin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-alpha 2 Macroglobulin antibody
See all alpha 2 Macroglobulin primary antibodies -
Description
Goat polyclonal to alpha 2 Macroglobulin -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Full length native protein (purified) corresponding to Human alpha 2 Macroglobulin aa 24-1474. (From Human plasma).
Sequence:SVSGKPQYMVLVPSLLHTETTEKGCVLLSYLNETVTVSASLESVRGNRSL FTDLEAENDVLHCVAFAVPKSSSNEEVMFLTVQVKGPTQEFKKRTTVMVK NEDSLVFVQTDKSIYKPGQTVKFRVVSMDENFHPLNELIPLVYIQDPKGN RIAQWQSFQLEGGLKQFSFPLSSEPFQGSYKVVVQKKSGGRTEHPFTVEE FVLPKFEVQVTVPKIITILEEEMNVSVCGLYTYGKPVPGHVTVSICRKYS DASDCHGEDSQAFCEKFSGQLNSHGCFYQQVKTKVFQLKRKEYEMKLHTE AQIQEEGTVVELTGRQSSEITRTITKLSFVKVDSHFRQGIPFFGQVRLVD GKGVPIPNKVIFIRGNEANYYSNATTDEHGLVQFSINTTNVMGTSLTVRV NYKDRSPCYGYQWVSEEHEEAHHTAYLVFSPSKSFVHLEPMSHELPCGHT QTVQAHYILNGGTLLGLKKLSFYYLIMAKGGIVRTGTHGLLVKQEDMKGH FSISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDVENCLANKVDLSFS PSQSLPASHAHLRVTAAPQSVCALRAVDQSVLLMKPDAELSASSVYNLLP EKDLTGFPGPLNDQDNEDCINRHNVYINGITYTPVSSTNEKDMYSFLEDM GLKAFTNSKIRKPKMCPQLQQYEMHGPEGLRVGFYESDVMGRGHARLVHV EEPHTETVRKYFPETWIWDLVVVNSAGVAEVGVTVPDTITEWKAGAFCLS EDAGLGISSTASLRAFQPFFVELTMPYSVIRGEAFTLKATVLNYLPKCIR VSVQLEASPAFLAVPVEKEQAPHCICANGRQTVSWAVTPKSLGNVNFTVS AEALESQELCGTEVPSVPEHGRKDTVIKPLLVEPEGLEKETTFNSLLCPS GGEVSEELSLKLPPNVVEESARASVSVLGDILGSAMQNTQNLLQMPYGCG EQNMVLFAPNIYVLDYLNETQQLTPEIKSKAIGYLNTGYQRQLNYKHYDG SYSTFGERYGRNQGNTWLTAFVLKTFAQARAYIFIDEAHITQALIWLSQR QKDNGCFRSSGSLLNNAIKGGVEDEVTLSAYITIALLEIPLTVTHPVVRN ALFCLESAWKTAQEGDHGSHVYTKALLAYAFALAGNQDKRKEVLKSLNEE AVKKDNSVHWERPQKPKAPVGHFYEPQAPSAEVEMTSYVLLAYLTAQPAP TSEDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHALSKYGAATFTRTG KAAQVTIQSSGTFSSKFQVDNNNRLLLQQVSLPELPGEYSMKVTGEGCVY LQTSLKYNILPEKEEFPFALGVQTLPQTCDEPKAHTSFQISLSVSYTGSR SASNMAIVDVKMVSGFIPLKPTVKMLERSNHVSRTEVSSNHVLIYLDKVS NQTLSLFFTVLQDVPVRDLKPAIVKVYDYYETDEFAIAEYNAPCSKDLGN A
Database link: P01023 -
Positive control
- Human alpha 2 Macroglobulin.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
ab181656 is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab181656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/20000. Predicted molecular weight: 163 kDa. |
Target
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Function
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. -
Tissue specificity
Secreted in plasma. -
Sequence similarities
Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. -
Developmental stage
Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2 Human
- Omim: 103950 Human
- SwissProt: P01023 Human
- Unigene: 212838 Human
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Alternative names
- A2m antibody
- A2MG_HUMAN antibody
- Alpha 2 M antibody
see all
Images
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Western blot - Anti-alpha 2 Macroglobulin antibody (ab181656)Anti-alpha 2 Macroglobulin antibody (ab181656) at 1/1000 dilution + Human alpha 2 Macroglobulin at 0.05 µg
Secondary
Peroxidase anti-Goat at 1/40000 dilution
Predicted band size: 163 kDa -
Western blot - Anti-alpha 2 Macroglobulin antibody (ab181656)All lanes : Anti-alpha 2 Macroglobulin antibody (ab181656) at 1/3000 dilution
Lane 1 : Alpha 2 Macroglobulin under reducing (R) conditions
Lane 2 : Alpha 2 Macroglobulin under non-reducing (NR) conditions
Lysates/proteins at 1 µg per lane.
Secondary
All lanes : Dylight 649 conjugated Donkey anti goat at 1/10000 dilution
Predicted band size: 163 kDaReduced samples of purified protein contained 4% BME and were boiled for 5 minutes.
Protocols
Datasheets and documents
References (0)
ab181656 has not yet been referenced specifically in any publications.
