Anti-alpha 2 Macroglobulin antibody (ab181656)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-alpha 2 Macroglobulin antibody
See all alpha 2 Macroglobulin primary antibodies -
Description
Goat polyclonal to alpha 2 Macroglobulin -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Full length native protein (purified) corresponding to Human alpha 2 Macroglobulin aa 24-1474. (From Human plasma).
Sequence:SVSGKPQYMVLVPSLLHTETTEKGCVLLSYLNETVTVSASLESVRGNRSL FTDLEAENDVLHCVAFAVPKSSSNEEVMFLTVQVKGPTQEFKKRTTVMVK NEDSLVFVQTDKSIYKPGQTVKFRVVSMDENFHPLNELIPLVYIQDPKGN RIAQWQSFQLEGGLKQFSFPLSSEPFQGSYKVVVQKKSGGRTEHPFTVEE FVLPKFEVQVTVPKIITILEEEMNVSVCGLYTYGKPVPGHVTVSICRKYS DASDCHGEDSQAFCEKFSGQLNSHGCFYQQVKTKVFQLKRKEYEMKLHTE AQIQEEGTVVELTGRQSSEITRTITKLSFVKVDSHFRQGIPFFGQVRLVD GKGVPIPNKVIFIRGNEANYYSNATTDEHGLVQFSINTTNVMGTSLTVRV NYKDRSPCYGYQWVSEEHEEAHHTAYLVFSPSKSFVHLEPMSHELPCGHT QTVQAHYILNGGTLLGLKKLSFYYLIMAKGGIVRTGTHGLLVKQEDMKGH FSISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDVENCLANKVDLSFS PSQSLPASHAHLRVTAAPQSVCALRAVDQSVLLMKPDAELSASSVYNLLP EKDLTGFPGPLNDQDNEDCINRHNVYINGITYTPVSSTNEKDMYSFLEDM GLKAFTNSKIRKPKMCPQLQQYEMHGPEGLRVGFYESDVMGRGHARLVHV EEPHTETVRKYFPETWIWDLVVVNSAGVAEVGVTVPDTITEWKAGAFCLS EDAGLGISSTASLRAFQPFFVELTMPYSVIRGEAFTLKATVLNYLPKCIR VSVQLEASPAFLAVPVEKEQAPHCICANGRQTVSWAVTPKSLGNVNFTVS AEALESQELCGTEVPSVPEHGRKDTVIKPLLVEPEGLEKETTFNSLLCPS GGEVSEELSLKLPPNVVEESARASVSVLGDILGSAMQNTQNLLQMPYGCG EQNMVLFAPNIYVLDYLNETQQLTPEIKSKAIGYLNTGYQRQLNYKHYDG SYSTFGERYGRNQGNTWLTAFVLKTFAQARAYIFIDEAHITQALIWLSQR QKDNGCFRSSGSLLNNAIKGGVEDEVTLSAYITIALLEIPLTVTHPVVRN ALFCLESAWKTAQEGDHGSHVYTKALLAYAFALAGNQDKRKEVLKSLNEE AVKKDNSVHWERPQKPKAPVGHFYEPQAPSAEVEMTSYVLLAYLTAQPAP TSEDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHALSKYGAATFTRTG KAAQVTIQSSGTFSSKFQVDNNNRLLLQQVSLPELPGEYSMKVTGEGCVY LQTSLKYNILPEKEEFPFALGVQTLPQTCDEPKAHTSFQISLSVSYTGSR SASNMAIVDVKMVSGFIPLKPTVKMLERSNHVSRTEVSSNHVLIYLDKVS NQTLSLFFTVLQDVPVRDLKPAIVKVYDYYETDEFAIAEYNAPCSKDLGN A
Database link: P01023 -
Positive control
- Human alpha 2 Macroglobulin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
ab181656 is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab181656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/2000 - 1/20000. Predicted molecular weight: 163 kDa. |
Target
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Function
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. -
Tissue specificity
Secreted in plasma. -
Sequence similarities
Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. -
Developmental stage
Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2 Human
- Omim: 103950 Human
- SwissProt: P01023 Human
- Unigene: 212838 Human
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Alternative names
- A2m antibody
- A2MG_HUMAN antibody
- Alpha 2 M antibody
see all
Images
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Western blot - Anti-alpha 2 Macroglobulin antibody (ab181656)Anti-alpha 2 Macroglobulin antibody (ab181656) at 1/1000 dilution + Human alpha 2 Macroglobulin at 0.05 µg
Secondary
Peroxidase anti-Goat at 1/40000 dilution
Predicted band size: 163 kDa -
Western blot - Anti-alpha 2 Macroglobulin antibody (ab181656)All lanes : Anti-alpha 2 Macroglobulin antibody (ab181656) at 1/3000 dilution
Lane 1 : Alpha 2 Macroglobulin under reducing (R) conditions
Lane 2 : Alpha 2 Macroglobulin under non-reducing (NR) conditions
Lysates/proteins at 1 µg per lane.
Secondary
All lanes : Dylight 649 conjugated Donkey anti goat at 1/10000 dilution
Predicted band size: 163 kDaReduced samples of purified protein contained 4% BME and were boiled for 5 minutes.
Datasheets and documents
References
ab181656 has not yet been referenced specifically in any publications.
