Key features and details
- Goat polyclonal to alpha 2 Macroglobulin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-alpha 2 Macroglobulin antibody
See all alpha 2 Macroglobulin primary antibodies
DescriptionGoat polyclonal to alpha 2 Macroglobulin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Full length native protein (purified) corresponding to Human alpha 2 Macroglobulin aa 24-1474. (From Human plasma).
SVSGKPQYMVLVPSLLHTETTEKGCVLLSYLNETVTVSASLESVRGNRSL FTDLEAENDVLHCVAFAVPKSSSNEEVMFLTVQVKGPTQEFKKRTTVMVK NEDSLVFVQTDKSIYKPGQTVKFRVVSMDENFHPLNELIPLVYIQDPKGN RIAQWQSFQLEGGLKQFSFPLSSEPFQGSYKVVVQKKSGGRTEHPFTVEE FVLPKFEVQVTVPKIITILEEEMNVSVCGLYTYGKPVPGHVTVSICRKYS DASDCHGEDSQAFCEKFSGQLNSHGCFYQQVKTKVFQLKRKEYEMKLHTE AQIQEEGTVVELTGRQSSEITRTITKLSFVKVDSHFRQGIPFFGQVRLVD GKGVPIPNKVIFIRGNEANYYSNATTDEHGLVQFSINTTNVMGTSLTVRV NYKDRSPCYGYQWVSEEHEEAHHTAYLVFSPSKSFVHLEPMSHELPCGHT QTVQAHYILNGGTLLGLKKLSFYYLIMAKGGIVRTGTHGLLVKQEDMKGH FSISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDVENCLANKVDLSFS PSQSLPASHAHLRVTAAPQSVCALRAVDQSVLLMKPDAELSASSVYNLLP EKDLTGFPGPLNDQDNEDCINRHNVYINGITYTPVSSTNEKDMYSFLEDM GLKAFTNSKIRKPKMCPQLQQYEMHGPEGLRVGFYESDVMGRGHARLVHV EEPHTETVRKYFPETWIWDLVVVNSAGVAEVGVTVPDTITEWKAGAFCLS EDAGLGISSTASLRAFQPFFVELTMPYSVIRGEAFTLKATVLNYLPKCIR VSVQLEASPAFLAVPVEKEQAPHCICANGRQTVSWAVTPKSLGNVNFTVS AEALESQELCGTEVPSVPEHGRKDTVIKPLLVEPEGLEKETTFNSLLCPS GGEVSEELSLKLPPNVVEESARASVSVLGDILGSAMQNTQNLLQMPYGCG EQNMVLFAPNIYVLDYLNETQQLTPEIKSKAIGYLNTGYQRQLNYKHYDG SYSTFGERYGRNQGNTWLTAFVLKTFAQARAYIFIDEAHITQALIWLSQR QKDNGCFRSSGSLLNNAIKGGVEDEVTLSAYITIALLEIPLTVTHPVVRN ALFCLESAWKTAQEGDHGSHVYTKALLAYAFALAGNQDKRKEVLKSLNEE AVKKDNSVHWERPQKPKAPVGHFYEPQAPSAEVEMTSYVLLAYLTAQPAP TSEDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHALSKYGAATFTRTG KAAQVTIQSSGTFSSKFQVDNNNRLLLQQVSLPELPGEYSMKVTGEGCVY LQTSLKYNILPEKEEFPFALGVQTLPQTCDEPKAHTSFQISLSVSYTGSR SASNMAIVDVKMVSGFIPLKPTVKMLERSNHVSRTEVSSNHVLIYLDKVS NQTLSLFFTVLQDVPVRDLKPAIVKVYDYYETDEFAIAEYNAPCSKDLGN A
Database link: P01023
- Human alpha 2 Macroglobulin.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
Purification notesab181656 is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Our Abpromise guarantee covers the use of ab181656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/20000. Predicted molecular weight: 163 kDa.|
FunctionIs able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Tissue specificitySecreted in plasma.
Sequence similaritiesBelongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
Developmental stageContrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
- Information by UniProt
- A2m antibody
- A2MG_HUMAN antibody
- Alpha 2 M antibody
Anti-alpha 2 Macroglobulin antibody (ab181656) at 1/1000 dilution + Human alpha 2 Macroglobulin at 0.05 µg
Peroxidase anti-Goat at 1/40000 dilution
Predicted band size: 163 kDa
All lanes : Anti-alpha 2 Macroglobulin antibody (ab181656) at 1/3000 dilution
Lane 1 : Alpha 2 Macroglobulin under reducing (R) conditions
Lane 2 : Alpha 2 Macroglobulin under non-reducing (NR) conditions
Lysates/proteins at 1 µg per lane.
All lanes : Dylight 649 conjugated Donkey anti goat at 1/10000 dilution
Predicted band size: 163 kDa
Reduced samples of purified protein contained 4% BME and were boiled for 5 minutes.
ab181656 has not yet been referenced specifically in any publications.