Recombinant
RabMAb

Recombinant Anti-alpha 2 Macroglobulin antibody [EPR4431] (ab133299)

Overview

  • Product name

    Anti-alpha 2 Macroglobulin antibody [EPR4431]
    See all alpha 2 Macroglobulin primary antibodies
  • Description

    Rabbit monoclonal [EPR4431] to alpha 2 Macroglobulin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human alpha 2 Macroglobulin (UniProt P01023).

  • Positive control

    • Human plasma lysate
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 163 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
    • Tissue specificity

      Secreted in plasma.
    • Sequence similarities

      Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
    • Developmental stage

      Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • A2m antibody
      • A2MG_HUMAN antibody
      • Alpha 2 M antibody
      • Alpha 2M antibody
      • Alpha-2-M antibody
      • Alpha-2-macroglobulin antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 antibody
      • CPAMD5 antibody
      • DKFZp779B086 antibody
      • FWP007 antibody
      • S863 7 antibody
      see all

    Images

    • Anti-alpha 2 Macroglobulin antibody [EPR4431] (ab133299) at 1/1000 dilution + Human plasma lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 163 kDa

    References

    ab133299 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you for your answer. We have added one unit of ab133299 to your pending order xx. I hope the customer will have good results with this new vial. Thank you for your help and cooperation. Please do not hesitate to contact us if you need anything further.

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