Recombinant
RabMAb

Recombinant Anti-alpha 2 Macroglobulin antibody [EPR4432] (ab109422)

Overview

  • Product name

    Anti-alpha 2 Macroglobulin antibody [EPR4432]
    See all alpha 2 Macroglobulin primary antibodies
  • Description

    Rabbit monoclonal [EPR4432] to alpha 2 Macroglobulin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human alpha 2 Macroglobulin aa 1300-1400. The exact sequence is proprietary.

  • Positive control

    • Human plasma lysate; Human liver tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 163 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
    • Tissue specificity

      Secreted in plasma.
    • Sequence similarities

      Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
    • Developmental stage

      Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • A2m antibody
      • A2MG_HUMAN antibody
      • Alpha 2 M antibody
      • Alpha 2M antibody
      • Alpha-2-M antibody
      • Alpha-2-macroglobulin antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 antibody
      • CPAMD5 antibody
      • DKFZp779B086 antibody
      • FWP007 antibody
      • S863 7 antibody
      see all

    Images

    • Anti-alpha 2 Macroglobulin antibody [EPR4432] (ab109422) at 1/1000 dilution + Human plasma lysate at 10 µg

      Predicted band size: 163 kDa

    • ab109422, at 1/500 dilution, staining alpha 2 Macroglobulin in paraffin-embedded Human liver by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab109422 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109422.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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