Recombinant Anti-alpha 2 Macroglobulin antibody [EPR4432] - BSA and Azide free (ab247855)
![Western blot - Anti-alpha 2 Macroglobulin antibody [EPR4432] - BSA and Azide free (ab247855)](https://www.abcam.com/ps/products/247/ab247855/Images/ab247855-402841-anti-alpha-2-macroglobulin-antibody-epr4432-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-alpha 2 Macroglobulin antibody [EPR4432] - BSA and Azide free (ab247855)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4432] to alpha 2 Macroglobulin - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-alpha 2 Macroglobulin antibody [EPR4432] - BSA and Azide free
See all alpha 2 Macroglobulin primary antibodies -
Description
Rabbit monoclonal [EPR4432] to alpha 2 Macroglobulin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247855 is the carrier-free version of ab109422.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4432 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 163 kDa.
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 163 kDa. |
Target
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Function
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. -
Tissue specificity
Secreted in plasma. -
Sequence similarities
Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. -
Developmental stage
Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2 Human
- Omim: 103950 Human
- SwissProt: P01023 Human
- Unigene: 212838 Human
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Alternative names
- A2m antibody
- A2MG_HUMAN antibody
- Alpha 2 M antibody
see all
Images
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Western blot - Anti-alpha 2 Macroglobulin antibody [EPR4432] - BSA and Azide free (ab247855)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2 Macroglobulin antibody [EPR4432] - BSA and Azide free (ab247855)](https://www.abcam.com/ps/products/247/ab247855/Images/ab247855-1-anti-alpha-2-macroglobulin-antibody-epr4432-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2 Macroglobulin antibody [EPR4432] - BSA and Azide free (ab247855)This data was developed using ab109422, the same antibody clone in a different buffer formulation.ab109422, at 1/500 dilution, staining alpha 2 Macroglobulin in paraffin-embedded Human liver by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Anti-alpha 2 Macroglobulin antibody [EPR4432] - BSA and Azide free (ab247855)](https://www.abcam.com/ps/products/247/ab247855/Images/ab247855-3-benefits-of-recombinant-antibodies.png)
Anti-alpha 2 Macroglobulin antibody [EPR4432] - BSA and Azide free (ab247855)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab247855 has not yet been referenced specifically in any publications.
