Recombinant
RabMAb

Anti-alpha 2 Macroglobulin antibody [EPSISR39] (ab156015)

Overview

  • Product name
    Anti-alpha 2 Macroglobulin antibody [EPSISR39]
    See all alpha 2 Macroglobulin primary antibodies
  • Description
    Rabbit monoclonal [EPSISR39] to alpha 2 Macroglobulin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human alpha 2 Macroglobulin (UniProt P01023).

  • Positive control
    • Human serum and plasma lysates; HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab156015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 163 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
    • Tissue specificity
      Secreted in plasma.
    • Sequence similarities
      Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
    • Developmental stage
      Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • A2m antibody
      • A2MG_HUMAN antibody
      • Alpha 2 M antibody
      • Alpha 2M antibody
      • Alpha-2-M antibody
      • Alpha-2-macroglobulin antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 antibody
      • CPAMD5 antibody
      • DKFZp779B086 antibody
      • FWP007 antibody
      • S863 7 antibody
      see all

    Images

    • All lanes : Anti-alpha 2 Macroglobulin antibody [EPSISR39] (ab156015) at 1/10000 dilution

      Lane 1 : Human serum lysate
      Lane 2 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 163 kDa

    • Immunofluorescent analysis of HepG2 cells labeling alpha 2 Macroglobulin with ab156015 at 1/100 dilution.

    References

    ab156015 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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