Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPSISR39] to alpha 2 Macroglobulin - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Human
Product nameAnti-alpha 2 Macroglobulin antibody [EPSISR39] - BSA and Azide free
See all alpha 2 Macroglobulin primary antibodies
DescriptionRabbit monoclonal [EPSISR39] to alpha 2 Macroglobulin - BSA and Azide free
Tested applicationsSuitable for: ICC, WBmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human alpha 2 Macroglobulin. The exact sequence is proprietary.
Database link: P01023
Ab249254 is the carrier-free version of ab156015. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab249254 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
Our Abpromise guarantee covers the use of ab249254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 163 kDa.|
FunctionIs able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Tissue specificitySecreted in plasma.
Sequence similaritiesBelongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
Developmental stageContrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
- Information by UniProt
- A2m antibody
- A2MG_HUMAN antibody
- Alpha 2 M antibody
All lanes : Anti-alpha 2 Macroglobulin antibody [EPSISR39] (ab156015) at 1/10000 dilution
Lane 1 : Human serum lysate
Lane 2 : Human plasma lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 163 kDa
This data was developed using ab156015, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab249254 has not yet been referenced specifically in any publications.