For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    alpha-2a-adrenergic-receptor-antibody-ab85570.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurotransmission Receptors / Channels GPCR Adrenergic Receptors
Share by email

Anti-alpha 2a Adrenergic Receptor antibody (ab85570)

  • Datasheet
Submit a review Q&A (3)References (4)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)

Key features and details

  • Rabbit polyclonal to alpha 2a Adrenergic Receptor
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Primary
Product image
Anti-alpha 1a Adrenergic Receptor/ADRA1A antibody [EPR9691(B)] (ab137123)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
Product image
Anti-beta 1 Adrenergic Receptor antibody (ab85037)

View more associated products

Overview

  • Product name

    Anti-alpha 2a Adrenergic Receptor antibody
    See all alpha 2a Adrenergic Receptor primary antibodies
  • Description

    Rabbit polyclonal to alpha 2a Adrenergic Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to Human alpha 2a Adrenergic Receptor aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab102061)

  • Positive control

    • Recombinant human alpha 2a Adrenergic Receptor protein (ab54291) can be used as a positive control in WB. This antibody gave a positive signal in Human pancreas tissue lysates.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Adrenergic Receptors
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab85570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 49 kDa).
Notes
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 49 kDa).

Target

  • Function

    Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2A sub-subfamily.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P08913 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 282135 Cow
    • Entrez Gene: 150 Human
    • Entrez Gene: 11551 Mouse
    • Entrez Gene: 399501 Pig
    • Entrez Gene: 25083 Rat
    • Omim: 104210 Human
    • SwissProt: Q28838 Cow
    • SwissProt: P08913 Human
    • SwissProt: Q01338 Mouse
    • SwissProt: P18871 Pig
    • SwissProt: P22909 Rat
    • Unigene: 249159 Human
    • Unigene: 235195 Mouse
    • Unigene: 170171 Rat
    see all
  • Alternative names

    • ADA2A_HUMAN antibody
    • ADRA2 antibody
    • ADRA2A antibody
    • ADRA2R antibody
    • ADRAR antibody
    • Adrenergic alpha 2A receptor antibody
    • Alpha 2 adrenergic receptor subtype C10 antibody
    • Alpha 2A adrenergic receptor antibody
    • Alpha 2AAR subtype C10 antibody
    • Alpha-2 adrenergic receptor subtype C10 antibody
    • alpha-2-adrenergic receptor, platelet type antibody
    • Alpha-2A adrenergic receptor antibody
    • Alpha-2A adrenoceptor antibody
    • Alpha-2A adrenoreceptor antibody
    • Alpha-2AAR antibody
    • ALPHA2AAR antibody
    • OTTHUMP00000058849 antibody
    • ZNF32 antibody
    see all

Images

  • Western blot - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
    Western blot - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
    Anti-alpha 2a Adrenergic Receptor antibody (ab85570) at 2 µg/ml + Human Pancreas at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Predicted band size: 49 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    Blocking buffer: 2% BSA

    Gel type: MOPS

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
    ICC/IF image of ab85570 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85570, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293 and HepG2 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
    IHC image of alpha 2a Adrenergic Receptor staining in human Pancreas FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85570, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (4)

Publishing research using ab85570? Please let us know so that we can cite the reference in this datasheet.

ab85570 has been referenced in 4 publications.

  • Liu X  et al. Distribution and relative expression of vasoactive receptors on arteries. Sci Rep 10:15383 (2020). PubMed: 32958803
  • Wang W  et al. a2A-Adrenergic Receptor Inhibits the Progression of Cervical Cancer Through Blocking PI3K/AKT/mTOR Pathway. Onco Targets Ther 13:10535-10546 (2020). PubMed: 33116632
  • Yu Z  et al. Single-Cell Transcriptomic Map of the Human and Mouse Bladders. J Am Soc Nephrol 30:2159-2176 (2019). PubMed: 31462402
  • Weng X  et al. Reduced mitochondrial response sensitivity is involved in the anti-apoptotic effect of dexmedetomidine pretreatment in cardiomyocytes. Int J Mol Med 41:2328-2338 (2018). PubMed: 29328437

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Question

Could you please make the blocking peptides available for ab8557 and ab45871?

Read More

Abcam community

Verified customer

Asked on May 07 2014

Answer

The blocking peptide for ab85570 is ab102061.

For ab45871, ab54291 can be used.

Read More

Jared Kaminski

Abcam Scientific Support

Answered on May 07 2014

Question

Could you check on the alignment of my protein and sequence of the peptide against which this antibody was raised?

Read More

Abcam community

Verified customer

Asked on Aug 24 2012

Answer

BLAST alignment of the immunogen sequence for ab65833, anti-alpha 2a Adrenergic Receptor, with your protein of interest returned a result of 81% identity (13/16).



Please let us know if you have any questions.

Read More

Abcam Scientific Support

Answered on Aug 24 2012

Question

IHC-P with human pancreas: good
with mouse and rat pancreas: faint specific staiing and high background
AR: pH6, pH9 (best), proteinase, none
Ab: 1/400 RT (best) and o/n (too much background)
block: serum-free, 0.25% casein
2nd: OK
block with 3% H2O2

Read More

Abcam community

Verified customer

Asked on May 21 2012

Answer

Thank you for your phone call.
Here is the information for the discount code for trying ab85570 in mouse and rat. Below that section, please see the information for the free of charge replacement.
DISCOUNT CODE: xxx
Expiration date: xxx
I am very pleased to hear you would like to accept our offer and test ab85570 in mouse/rat. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse/rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

===== FREE of CHARGE REPLACEMENT =====

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab85570.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

Read More

Abcam Scientific Support

Answered on May 21 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.