Anti-alpha 2a Adrenergic Receptor antibody (ab85570)

Submit a review Q&A (3)References (1)

Key features and details

  • Rabbit polyclonal to alpha 2a Adrenergic Receptor
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-alpha 2a Adrenergic Receptor antibody
    See all alpha 2a Adrenergic Receptor primary antibodies

  • Description

    Rabbit polyclonal to alpha 2a Adrenergic Receptor

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig

  • Immunogen

    Synthetic peptide corresponding to Human alpha 2a Adrenergic Receptor aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab102061)

  • Positive control

    • Recombinant human alpha 2a Adrenergic Receptor protein (ab54291) can be used as a positive control in WB. This antibody gave a positive signal in Human small intestine and Human pancreas tissue lysates.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 49 kDa).

Target

  • Function

    Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2A sub-subfamily.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ADA2A_HUMAN antibody
    • ADRA2 antibody
    • ADRA2A antibody
    • ADRA2R antibody
    • ADRAR antibody
    • Adrenergic alpha 2A receptor antibody
    • Alpha 2 adrenergic receptor subtype C10 antibody
    • Alpha 2A adrenergic receptor antibody
    • Alpha 2AAR subtype C10 antibody
    • Alpha-2 adrenergic receptor subtype C10 antibody
    • alpha-2-adrenergic receptor, platelet type antibody
    • Alpha-2A adrenergic receptor antibody
    • Alpha-2A adrenoceptor antibody
    • Alpha-2A adrenoreceptor antibody
    • Alpha-2AAR antibody
    • ALPHA2AAR antibody
    • OTTHUMP00000058849 antibody
    • ZNF32 antibody
    see all

Images

  • Western blot - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
    Western blot - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
    All lanes : Anti-alpha 2a Adrenergic Receptor antibody (ab85570) at 1 µg/ml

    Lane 1 : Human pancreas tissue lysate - total protein (ab29816)
    Lane 2 : Human small intestine tissue lysate - total protein (ab29276)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    Alpha 2a Adrenergic Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
    ICC/IF image of ab85570 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85570, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293 and HepG2 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2a Adrenergic Receptor antibody (ab85570)
    IHC image of alpha 2a Adrenergic Receptor staining in human Pancreas FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85570, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

References (1)

Publishing research using ab85570? Please let us know so that we can cite the reference in this datasheet.

ab85570 has been referenced in 1 publication.

  • Weng X  et al. Reduced mitochondrial response sensitivity is involved in the anti-apoptotic effect of dexmedetomidine pretreatment in cardiomyocytes. Int J Mol Med 41:2328-2338 (2018). PubMed: 29328437

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

Could you please make the blocking peptides available for ab8557 and ab45871?

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Answer

The blocking peptide for ab85570 is ab102061.

For ab45871, ab54291 can be used.

Read More

Question

Could you check on the alignment of my protein and sequence of the peptide against which this antibody was raised?

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Answer

BLAST alignment of the immunogen sequence for ab65833, anti-alpha 2a Adrenergic Receptor, with your protein of interest returned a result of 81% identity (13/16).



Please let us know if you have any questions.

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Question

IHC-P with human pancreas: good
with mouse and rat pancreas: faint specific staiing and high background
AR: pH6, pH9 (best), proteinase, none
Ab: 1/400 RT (best) and o/n (too much background)
block: serum-free, 0.25% casein
2nd: OK
block with 3% H2O2

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Answer

Thank you for your phone call.
Here is the information for the discount code for trying ab85570 in mouse and rat. Below that section, please see the information for the free of charge replacement.
DISCOUNT CODE: xxx
Expiration date: xxx
I am very pleased to hear you would like to accept our offer and test ab85570 in mouse/rat. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse/rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

===== FREE of CHARGE REPLACEMENT =====

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab85570.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

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