Anti-alpha 2a Adrenergic Receptor antibody (ab92650)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-alpha 2a Adrenergic Receptor antibody
See all alpha 2a Adrenergic Receptor primary antibodies -
Description
Rabbit polyclonal to alpha 2a Adrenergic Receptor -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Guinea pig, Cow, Cat, Pig, Pufferfish, Zebrafish
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Immunogen
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Positive control
- Rat spinal cord dorsal horn, ventral horn motor neurons and retina.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab92650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Application notes
IHC-P: 1/100 - 1/500 for 18-24 hours at 4ºC.
Tested on perfusion fixed tissue, using calcium-free Tyrode’s solution, PFA-picric acid fixative, and 10% sucrose in PBS as a cryo-protectant.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tested on perfusion fixed tissue, using calcium-free Tyrode’s solution, PFA-picric acid fixative, and 10% sucrose in PBS as a cryo-protectant.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2A sub-subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 282135 Cow
- Entrez Gene: 150 Human
- Entrez Gene: 11551 Mouse
- Entrez Gene: 399501 Pig
- Entrez Gene: 25083 Rat
- Entrez Gene: 266750 Zebrafish
- Omim: 104210 Human
- SwissProt: Q27RP4 Cat
see all -
Alternative names
- ADA2A_HUMAN antibody
- ADRA2 antibody
- ADRA2A antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2a Adrenergic Receptor antibody (ab92650)ab92650 at 1/100 dilution staining alpha 2a Adrenergic Receptor in Rat spinal cord dorsal horn tissue by indirect immunofluorescence.
Protocols
Datasheets and documents
References
ab92650 has not yet been referenced specifically in any publications.
