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Neuroscience Neurotransmission Receptors / Channels GPCR Adrenergic Receptors
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Anti-alpha 2a Adrenergic Receptor antibody (ab92650)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2a Adrenergic Receptor antibody (ab92650)

    Key features and details

    • Rabbit polyclonal to alpha 2a Adrenergic Receptor
    • Suitable for: IHC-P
    • Reacts with: Rat
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Recombinant human alpha 2a Adrenergic Receptor protein (ab54291)

    View more associated products

    Overview

    • Product name

      Anti-alpha 2a Adrenergic Receptor antibody
      See all alpha 2a Adrenergic Receptor primary antibodies
    • Description

      Rabbit polyclonal to alpha 2a Adrenergic Receptor
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      IHC-P
      Rat
      See all applications and species data
    • Immunogen

      Synthetic peptide:

      KASRWRGRGNREKR

      with a Cysteine residue linker
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Rat spinal cord dorsal horn, ventral horn motor neurons and retina.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurotransmission
      • Receptors / Channels
      • GPCR
      • Adrenergic Receptors
      • Signal Transduction
      • Signaling Pathway
      • G Protein Signaling
      • GPCR

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Immunizing Peptide (Blocking)

      • alpha 2a Adrenergic Receptor peptide (ab195355)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human alpha 2a Adrenergic Receptor protein (ab54291)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab92650 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Rat
    All applications
    Guinea pig
    Cow
    Cat
    Pig
    Pufferfish
    Zebrafish
    Application Abreviews Notes
    IHC-P
    Notes
  • Application notes
    IHC-P: 1/100 - 1/500 for 18-24 hours at 4ºC.
    Tested on perfusion fixed tissue, using calcium-free Tyrode’s solution, PFA-picric acid fixative, and 10% sucrose in PBS as a cryo-protectant.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2A sub-subfamily.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P08913 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 282135 Cow
      • Entrez Gene: 399501 Pig
      • Entrez Gene: 25083 Rat
      • Entrez Gene: 266750 Zebrafish
      • SwissProt: Q27RP4 Cat
      • SwissProt: Q28838 Cow
      • SwissProt: P18871 Pig
      • SwissProt: P22909 Rat
      • SwissProt: Q1L8Y8 Zebrafish
      • SwissProt: Q90WY4 Zebrafish
      • Unigene: 170171 Rat
      • Unigene: 30878 Zebrafish
      see all
    • Alternative names

      • ADA2A_HUMAN antibody
      • ADRA2 antibody
      • ADRA2A antibody
      • ADRA2R antibody
      • ADRAR antibody
      • Adrenergic alpha 2A receptor antibody
      • Alpha 2 adrenergic receptor subtype C10 antibody
      • Alpha 2A adrenergic receptor antibody
      • Alpha 2AAR subtype C10 antibody
      • Alpha-2 adrenergic receptor subtype C10 antibody
      • alpha-2-adrenergic receptor, platelet type antibody
      • Alpha-2A adrenergic receptor antibody
      • Alpha-2A adrenoceptor antibody
      • Alpha-2A adrenoreceptor antibody
      • Alpha-2AAR antibody
      • ALPHA2AAR antibody
      • OTTHUMP00000058849 antibody
      • ZNF32 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2a Adrenergic Receptor antibody (ab92650)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2a Adrenergic Receptor antibody (ab92650)
      ab92650 at 1/100 dilution staining alpha 2a Adrenergic Receptor in Rat spinal cord dorsal horn tissue by indirect immunofluorescence.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab92650? Please let us know so that we can cite the reference in this datasheet.

    ab92650 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Western blot abreview for Anti-alpha 2a Adrenergic Receptor antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (crypts and villi)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    crypts and villi
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Ms. Melissa Montrose

    Verified customer

    Submitted Oct 01 2019

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