Overview

  • Product name
    Anti-alpha 2C Adrenergic Receptor antibody [N330A/80]
    See all alpha 2C Adrenergic Receptor primary antibodies
  • Description
    Mouse monoclonal [N330A/80] to alpha 2C Adrenergic Receptor
  • Host species
    Mouse
  • Specificity
    ab167433 does not cross-react with other adrenergic receptors.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Guinea pig
  • Immunogen

    Synthetic peptide, corresponding to C terminal amino acids 442-462 of Human alpha 2C Adrenergic Receptor (UniProt P18825).

  • Positive control
    • COS cells transiently transfected with HA-tagged alpha 2C Adrenergic Receptor lysate.
  • General notes

    The clone number has been updated from S330A-80 to N330A/80, both clone numbers name the same antibody clone.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab167433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 50 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    The alpha 2C Adrenergic Receptor controls the release of neurotransmitter from central adrenergic neurons and from sympathetic nerves in the heart. This receptor also plays a role in cognitive and behavioral function. Two variants are produced by alternative splicing. Alpha 2C Adrenergic Receptor has been reported extensively in the brain. Blood, corpus cavermosum, kidney, and heart tissues have also been reported to express the protein.
  • Cellular localization
    Cell membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • Adra 2c antibody
    • ADRA2C antibody
    • ADRA2L2 antibody
    • ADRA2RL2 antibody
    • ADRARL2 antibody
    • Adrenergic alpha 2C receptor antibody
    • Adrenoceptor alpha 2C antibody
    • Alpha 2C adrenergic receptor antibody
    • Alpha 2C adrenergic receptor subtype C4 antibody
    • Alpha 2C adrenoceptor antibody
    • Alpha 2C adrenoreceptor antibody
    • Alpha 2CAR antibody
    • Alpha-2-adrenergic receptor, renal type antibody
    • alpha2 AR C4 antibody
    • alpha2 C4 antibody
    • alpha2C antibody
    • ALPHA2CAR antibody
    • alpha2cii-adrenergic receptor antibody
    • Subtype alpha2 C4 antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE cells labeling alpha 2C Adrenergic Receptor using ab167433 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/100 dilution incubated for 1 hour at room temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).

  • Anti-alpha 2C Adrenergic Receptor antibody [N330A/80] (ab167433) at 1/200 dilution + Monkey COS cells transfected with HA-tagged alpha 2C Adrenergic Receptor

    Secondary
    Goat anti-mouse IgG at 1/1000 dilution

    Predicted band size: 50 kDa


    Exposure time: 6 minutes


    Membrane was blocked with 2% BSA and 2% Skim Milk in 1X TBST.

References

This product has been referenced in:
  • Albee LJ  et al. a1-Adrenergic Receptors Function Within Hetero-Oligomeric Complexes With Atypical Chemokine Receptor 3 and Chemokine (C-X-C motif) Receptor 4 in Vascular Smooth Muscle Cells. J Am Heart Assoc 6:N/A (2017). Read more (PubMed: 28862946) »
  • Tripathi A  et al. Heteromerization of chemokine (C-X-C motif) receptor 4 with a1A/B-adrenergic receptors controls a1-adrenergic receptor function. Proc Natl Acad Sci U S A 112:E1659-68 (2015). Read more (PubMed: 25775528) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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