Overview

  • Product name

    Anti-alpha 2u-globulin antibody [1D11]
    See all alpha 2u-globulin primary antibodies
  • Description

    Mouse monoclonal [1D11] to alpha 2u-globulin
  • Host species

    Mouse
  • Specificity

    ab27160 recognises alpha 2-microglobulin.
  • Tested applications

    Suitable for: Sandwich ELISA, ELISA, WBmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    Full length native protein (purified) corresponding to Rat alpha 2u-globulin .

Properties

Applications

Our Abpromise guarantee covers the use of ab27160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse Monoclonal [33B10] to alpha 2u-globulin (ab27161).
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.

Target

  • Relevance

    Alpha 2 microglobulin is part of the lipocalin family. There are many different isoforms which exhibit extensive homology. It is a major urinary protein in male rats and is synthesised and secreted by hepatic parenchymal cells. Alpha 2 microglobulin is also known as MUP (major urinary protein) and is a very important allergen because it constitutes approximately 30% of the total protein content excreted in the urine. Rats are the most frequently used laboratory animals and allergy to them constitutes an occupational disease. Approximately 20 % of the personal engaged in work with laboratory animals have acquired symptoms of allergy.
  • Cellular localization

    Cytoplasmic and Secreted
  • Database links

  • Alternative names

    • Allergen Rat n 1 antibody
    • Alpha (2) euglobulin antibody
    • Alpha 2u globulin antibody
    • Alpha2mu globulin antibody
    • Alpha2u antibody
    • Major urinary protein antibody
    • MUP antibody
    • PGCL1 antibody
    • Rat n1 antibody
    see all

References

ab27160 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
Yes - 3% H2O2
Specification
Kidney
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Apr 29 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Male Kidney)
Antigen retrieval step
None
Permeabilization
No
Specification
Male Kidney
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde

Ms. Katie Belsham

Verified customer

Submitted Nov 09 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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