This antibody gave a positive signal in the following tissue lysates: Rat Brain; Rat Cerebellum; Rat Cortex; Mouse Brain.
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pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 111 kDa).
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Involvement in disease
Dystonia 12 Alternating hemiplegia of childhood 2 Cerebellar ataxia, areflexia, pes cavus, optic atrophy, and sensorineural hearing loss
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
Please note that lane 4 (Rat Kidney tissue lysate) represents a Negative control for alpha 3 Sodium Potassium ATPase.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab150287 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.