Anti-alpha Defensin 5 antibody [8C8] (ab90802)
Key features and details
- Mouse monoclonal [8C8] to alpha Defensin 5
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2b
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-alpha Defensin 5 antibody [8C8]
See all alpha Defensin 5 primary antibodies -
Description
Mouse monoclonal [8C8] to alpha Defensin 5 -
Host species
Mouse -
Specificity
ab90802 recognizes the propeptide and the partially processed forms (aa 36-94 and 56-94), but it hardly recognizes mature peptide. No cross reactivity with HD6, lysozyme, or sPLA2 has be detected. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee -
Immunogen
Recombinant fragment corresponding to Human alpha Defensin 5 aa 20-94.
Sequence:ESLQERADEATTQKQSGEDNQDLAISFAGNGLSALRTSGSQARATCYCRT GRCATRESLSGVCEISGRLYRLCCR
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab90802 was filtered through a 0.2 µm filter. -
Clonality
Monoclonal -
Clone number
8C8 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab90802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (2) |
1/50.
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Notes |
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IHC-P
1/50. |
Target
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Function
Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing. -
Tissue specificity
Paneth cells of the small intestine (at protein level). -
Sequence similarities
Belongs to the alpha-defensin family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. Cytoplasmic vesicle > secretory vesicle. Peptides HD5(20-94), HD5(23-94) and HD5(29-94) are found within tissues, HD5(20-94) being the predominant intracellular form. Peptides HD5(56-94) and HD5(63-94) are found in the extracellular milieu, HD5(63-94) being the most abundant form. - Information by UniProt
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Database links
- Entrez Gene: 463972 Chimpanzee
- Entrez Gene: 1670 Human
- Omim: 600472 Human
- SwissProt: Q5G861 Chimpanzee
- SwissProt: Q01523 Human
- Unigene: 655233 Human
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Alternative names
- alpha 5 antibody
- DEF5 antibody
- DEF5_HUMAN antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab90802 has been referenced in 2 publications.
- Liszt KI et al. Human intestinal bitter taste receptors regulate innate immune responses and metabolic regulators in obesity. J Clin Invest 132:N/A (2022). PubMed: 34784295
- Spencer JD et al. Human alpha defensin 5 expression in the human kidney and urinary tract. PLoS One 7:e31712 (2012). IHC ; Human . PubMed: 22359618