Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing.
Paneth cells of the small intestine (at protein level).
Belongs to the alpha-defensin family.
Secreted. Cytoplasmic vesicle > secretory vesicle. Peptides HD5(20-94), HD5(23-94) and HD5(29-94) are found within tissues, HD5(20-94) being the predominant intracellular form. Peptides HD5(56-94) and HD5(63-94) are found in the extracellular milieu, HD5(63-94) being the most abundant form.
Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling alpha 5 Defensin with ab180515 at 1/4000 dilution. Anti-rabbit HRP secondary antibody was used and counter staining with Hematoxylin performed.