Recombinant Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14125(B)] to alpha A Crystallin/CRYAA
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)]
See all alpha A Crystallin/CRYAA primary antibodies -
Description
Rabbit monoclonal [EPR14125(B)] to alpha A Crystallin/CRYAA -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Recombinant human alpha A Crystallin/CRYAA protein (ab48778) can be used as a positive control in WB. Rat and Mouse eyeball. Y79 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14125(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/2000. Predicted molecular weight: 20 kDa.
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ICC/IF |
1/500.
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Notes |
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WB
1/1000 - 1/2000. Predicted molecular weight: 20 kDa. |
ICC/IF
1/500. |
Target
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Function
May contribute to the transparency and refractive index of the lens. -
Involvement in disease
Defects in CRYAA are a cause of cataract autosomal dominant (ADC) [MIM:604219]. Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract is the most common treatable cause of visual disability in childhood. -
Sequence similarities
Belongs to the small heat shock protein (HSP20) family. -
Post-translational
modificationsO-glycosylated; contains N-acetylglucosamine side chains.
Deamidation of Asn-101 in lens occurs mostly during the first 30 years of age, followed by a small additional amount of deamidation (approximately 5%) during the next approximately 38 years, resulting in a maximum of approximately 50% deamidation during the lifetime of the individual.
Phosphorylation on Ser-122 seems to be developmentally regulated. Absent in the first months of life, it appears during the first 12 years of human lifetime. The relative amount of phosphorylated form versus unphosphorylated form does not change over the lifetime of the individual. -
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. - Information by UniProt
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Database links
- Entrez Gene: 1409 Human
- Entrez Gene: 12954 Mouse
- Entrez Gene: 24273 Rat
- Omim: 123580 Human
- SwissProt: P02489 Human
- SwissProt: P24622 Mouse
- SwissProt: P24623 Rat
- Unigene: 184085 Human
see all -
Alternative names
- Acry 1 antibody
- Alpha crystallin A chain antibody
- Alpha-crystallin A chain antibody
see all
Images
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Immunofluorescence analysis of Y79 cells fixed with acetone labeling alpha A Crystallin/CRYAA with ab181866 at 1/500 dilution followed by Goat anti rabbit IgG(Alexa Fluor®555) at 1/200 dilution and counterstained with Dapi.
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All lanes : Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866) at 1/2000 dilution
Lane 1 : Rat eyeball
Lane 2 : Mouse eyeball
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab181866 has been referenced in 3 publications.
- Zhao Z et al. Structural and functional analysis of SNP rs76740365 G>A in exon-3 of the alpha A-crystallin gene in lens epithelial cells. Mol Vis 28:317-330 (2022). PubMed: 36338667
- Xie X et al. Up-Regulation of GATA4 Regulates Human Lens Epithelial Cell Function in Age-Related Cataract. Ophthalmic Res 63:564-571 (2020). PubMed: 32305986
- Liu X et al. Long Non-Coding RNA H19 Regulates Human Lens Epithelial Cells Function. Cell Physiol Biochem 50:246-260 (2018). PubMed: 30286462