Product nameAnti-alpha Adducin (phospho S436) antibody [EPR2176]
See all alpha Adducin primary antibodies
DescriptionRabbit monoclonal [EPR2176] to alpha Adducin (phospho S436)
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
A phospho specific peptide corresponding to residues surrounding Serine 436 of Human alpha Adducin.
- HeLa cell lysate.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab125081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 81 kDa).|
FunctionMembrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Tissue specificityExpressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
Sequence similaritiesBelongs to the aldolase class II family. Adducin subfamily.
DomainEach subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
Cellular localizationCytoplasm > cytoskeleton. Cell membrane.
- Information by UniProt
- ADD A antibody
- ADD1 antibody
- ADDA antibody
All lanes : Anti-alpha Adducin (phospho S436) antibody [EPR2176] (ab125081) at 1/1000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 81 kDa
The bands shown at the bottom of the gel are Beta Tubulin.
ab125081 has not yet been referenced specifically in any publications.