Recombinant Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)
![Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)](https://www.abcam.com/ps/products/168/ab168381/Images/ab168381-477471-168381Alphasynucleinphospho-S129westernblot.jpg "Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MJF-R13 (8-8)] to Alpha-synuclein (phospho S129)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)]
See all Alpha-synuclein primary antibodies -
Description
Rabbit monoclonal [MJF-R13 (8-8)] to Alpha-synuclein (phospho S129) -
Host species
Rabbit -
Specificity
ab168381 only detects alpha Synuclein phosphorylated on Ser129. -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Alpha-synuclein (phospho S129). The exact sequence is proprietary.
Database link: P37840 -
Positive control
- Recombinant alpha-synuclein, expressed in BL21 bacterial cells in the presence of Human Polo-Like Kinase 2; HEK whole cell lysates, stably-transfected with Polo-Like Kinase 2 and alpha Synuclein.
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General notes
Alpha-synuclein was the first gene to be linked to Parkinson’s disease (PD) and remains the most promising link to PD pathogenesis, where there is genetic evidence that it may play a causal role. In the brain, alpha-synuclein is concentrated in presynaptic nerve terminals. The deposition of the abundant presynaptic brain protein alpha-synuclein as fibrillary aggregates in neurons or glial cells is a hallmark lesion in a subset of neurodegenerative disorders. These disorders include Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy, collectively referred to as synucleinopathies. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin.
Recent studies also indicate that alpha-synuclein undergoes post-translational modification. Though the role of many of these modifications is still under investigation, phosphorylation at Serine 129 may affect alpha-synuclein aggregations and may also serve as marker of disease pathogenesis. With the advent of this phospho-specific Serine 129 antibody, The Michael J. Fox Foundation hopes to ensure that the putative role of this modification can be further examined by all researchers.This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This antibody was developed with support from The Michael J. Fox Foundation.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MJF-R13 (8-8) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/1000. Predicted molecular weight: 14 kDa.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 14 kDa. |
Target
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Function
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. -
Tissue specificity
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals. -
Involvement in disease
Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body -
Sequence similarities
Belongs to the synuclein family. -
Domain
The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments. -
Post-translational
modificationsPhosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. -
Cellular localization
Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons. - Information by UniProt
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Database links
- Entrez Gene: 6622 Human
- Omim: 163890 Human
- SwissProt: P37840 Human
- Unigene: 21374 Human
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Alternative names
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
see all
Images
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Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)All lanes : Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)
Lane 1 : In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of PLK2 (Polo-like kinase 2) but absence of ATP
Lane 2 : In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of ATP but absence of PLK2 (Polo-like kinase 2)
Lane 3 : In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of PLK2 (Polo-like kinase 2) and ATP
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?Blocking and Diluting buffer and concentration - 5% NFDM/TBST
Exposure time: 5 seconds
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![Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)](https://www.abcam.com/ps/products/168/ab168381/Images/ab168381-332420-anti-alpha-synuclein-phospho-s129-antibody-mjf-r13-8-8-western-blot.jpg)
Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)Image courtesy of Mr. Chun Chau SUNGAnti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381) at 1/2000 dilution + Mouse cortical neuron lysate at 30 µg
Secondary
Goat anti-rabbit IgG (H+L) HRP
Developed using the ECL technique.
Predicted band size: 14 kDa
Exposure time: 5 minutesLysate prepared in PBS + 1% Triton X-100. Membrane fixed with 0.4% PFA in PBS for 30 min prior to blocking.
Primary incubation for 12 hours at 4°C.
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![Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)](https://www.abcam.com/ps/products/168/ab168381/Images/ab168381-166676-anti-alpha-synuclein-phospho-s129-antibody-mjf-r13-8-8-western-blot.jpg)
Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)This image is courtesy of Drs. Shyra Gardai and Jennifer Johnston (Elan Pharmaceuticals)All lanes : Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381) at 1/1000 dilution
Lane 1 : Recombinant alpha Synuclein expressed in BL21 bacterial cells
Lane 2 : Recombinant alpha Synuclein expressed in BL21 bacterial cells, in the presence of Human Polo-Like Kinase 2
Lane 3 : HEK whole cell lysates, stably-transfected with Polo-Like Kinase 2 and alpha Synuclein
Predicted band size: 14 kDa -
![Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)](https://www.abcam.com/ps/products/168/ab168381/Images/ab168381-3-benefits-of-recombinant-antibodies.png)
Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab168381 has been referenced in 25 publications.
- Tanei ZI et al. Lewy pathology of the esophagus correlates with the progression of Lewy body disease: a Japanese cohort study of autopsy cases. Acta Neuropathol 141:25-37 (2021). PubMed: 33150517
- Baxter PS et al. Targeted de-repression of neuronal Nrf2 inhibits a-synuclein accumulation. Cell Death Dis 12:218 (2021). PubMed: 33637689
- Kim K et al. Reduced Interaction of Aggregated a-Synuclein and VAMP2 by Environmental Enrichment Alleviates Hyperactivity and Anxiety in a Model of Parkinson's Disease. Genes (Basel) 12:N/A (2021). PubMed: 33801790
- Henderson MX et al. Glucocerebrosidase Activity Modulates Neuronal Susceptibility to Pathological a-Synuclein Insult. Neuron 105:822-836.e7 (2020). PubMed: 31899072
- Stoyka LE et al. Behavioral defects associated with amygdala and cortical dysfunction in mice with seeded a-synuclein inclusions. Neurobiol Dis 134:104708 (2020). PubMed: 31837424