Product nameAnti-Alpha A + alpha B Crystallin antibody
See all Alpha A + alpha B Crystallin primary antibodies
DescriptionRabbit polyclonal to Alpha A + alpha B Crystallin
Specificityab28163 recognises alpha A and alpha B crystallin proteins. There is no reactivity with beta crystallin.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Mouse, Cow, Human
- Bovine alpha Crystallin Protein, Bovine alpha A Crystallin Protein, Bovine alpha B Crystallin Protein
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab28163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 20 kDa.
This dilution was sufficient for detection of alpha A/beta B-crystallin in 20µg of alpha A-crystallin protein by colorimetric immunoblot analysis. Goat anti-Rabbit IgG-AP as secondary antibody.
|ELISA||Use at an assay dependent concentration.|
RelevanceCrystallins are separated into two classes: taxon specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Alpha crystallins are composed of two gene products: alpha A and alpha B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture.
- Alpha crystallin A chain antibody
- Alpha crystallin B chain antibody
- CRYA1 antibody
All lanes : Anti-Alpha A + alpha B Crystallin antibody (ab28163) at 1/1000 dilution
Lane 1 : Tissue lysates prepared from mouse heart
Lane 2 : Cell lysates prepared from Hela cells
Lane 3 : Crystallin alpha B native bovine protein
Lane 4 : Crystallin alpha native bovine protein
Lane 5 : Molecular weight marker
Predicted band size: 20 kDa
ab28163 has not yet been referenced specifically in any publications.