The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 20 kDa.
This dilution was sufficient for detection of alpha A/beta B-crystallin in 20µg of alpha A-crystallin protein by colorimetric immunoblot analysis. Goat anti-Rabbit IgG-AP as secondary antibody.
Use at an assay dependent concentration.
Crystallins are separated into two classes: taxon specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens.
Alpha crystallins are composed of two gene products: alpha A and alpha B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein family.
They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture.
Western blot - Anti-Alpha A + alpha B Crystallin antibody (ab28163)
All lanes : Anti-Alpha A + alpha B Crystallin antibody (ab28163) at 1/1000 dilution
Lane 1 : Tissue lysates prepared from mouse heart Lane 2 : Cell lysates prepared from Hela cells Lane 3 : Crystallin alpha B native bovine protein Lane 4 : Crystallin alpha native bovine protein Lane 5 : Molecular weight marker