Overview

  • Product name

    Anti-alpha A Crystallin/CRYAA antibody
    See all alpha A Crystallin/CRYAA primary antibodies
  • Description

    Rabbit polyclonal to alpha A Crystallin/CRYAA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human alpha A Crystallin/CRYAA aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: P02489

  • Positive control

    • This antibody gave a positive signal in Human Brain and Spinal Cord tissue lysates.
  • General notes

     This product was previously labelled as alpha A Crystallin

     

Properties

Applications

Our Abpromise guarantee covers the use of ab139503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

Target

  • Function

    May contribute to the transparency and refractive index of the lens.
  • Involvement in disease

    Defects in CRYAA are a cause of cataract autosomal dominant (ADC) [MIM:604219]. Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract is the most common treatable cause of visual disability in childhood.
  • Sequence similarities

    Belongs to the small heat shock protein (HSP20) family.
  • Post-translational
    modifications

    O-glycosylated; contains N-acetylglucosamine side chains.
    Deamidation of Asn-101 in lens occurs mostly during the first 30 years of age, followed by a small additional amount of deamidation (approximately 5%) during the next approximately 38 years, resulting in a maximum of approximately 50% deamidation during the lifetime of the individual.
    Phosphorylation on Ser-122 seems to be developmentally regulated. Absent in the first months of life, it appears during the first 12 years of human lifetime. The relative amount of phosphorylated form versus unphosphorylated form does not change over the lifetime of the individual.
  • Cellular localization

    Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acry 1 antibody
    • Alpha crystallin A chain antibody
    • Alpha-crystallin A chain antibody
    • CRYA 1 antibody
    • CRYA1 antibody
    • CRYAA antibody
    • CRYAA_HUMAN antibody
    • Crystallin alpha 1 antibody
    • Crystallin alpha A antibody
    • Heat shock protein beta 4 antibody
    • Heat shock protein beta-4 antibody
    • HSPB 4 antibody
    • HspB4 antibody
    • short form antibody
    • Zonular Central Nuclear Cataract antibody
    see all

Images

  • All lanes : Anti-alpha A Crystallin/CRYAA antibody (ab139503) at 1 µg/ml

    Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Spinal Cord (Human) Tissue Lysate - adult normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Donkey Anti-Rabbit IgG H&L preadsorbed (ab97081) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 20 kDa
    Observed band size: 20 kDa
    Additional bands at: 28 kDa (possible non-specific binding)


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab139503 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab139503 has not yet been referenced specifically in any publications.

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