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Can this ab5595 detect the phospho and non-phospho proteins?
Asked on Jan 14 2009
Thanks for your email. I have checked the product file and it clearly states that this antibody does not detect the non-phosphorylated version of this protein, but there is no data in the file to indicate how this was determined. I looked up several early references that describe the production of this antibody and I'm not sure why it only recognizes the phosphorylated form of the alpha A crystalline. The paper that describes the original production of the antibody (Kato et al. Biochimica et Biophysica Acta 1991 v1080 p173-180) describes the alpha-A crystallin and how it is split into alpha-A1 and alpha-A2 forms, the A1 form being phosphorylated and A2 being non-phosphorylated. Apparently the A1 form was used as the immunogen for the production of PA1-009. The A1 form is phosphorylated at serine residues exclusively but as this is a polyclonal antibody I would expect other epitopes to exist that are not based on phosphorylation. I can no longer contact the person that generated this antibody, but I looked through our records and saw the statement that the antibody recognizes only the phosphorylated form several times, so I can only assume this has been well characterized. Sorry I don't have a more definitive answer for you but I hope this helps.
Answered on Jan 14 2009