Key features and details
- Rabbit polyclonal to alpha A Crystallin/CRYAA
- Suitable for: WB
- Reacts with: Cow, Human
- Isotype: IgG
Product nameAnti-alpha A Crystallin/CRYAA antibody
See all alpha A Crystallin/CRYAA primary antibodies
DescriptionRabbit polyclonal to alpha A Crystallin/CRYAA
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Cow, Human
Predicted to work with: Chicken
Synthetic peptide corresponding to Human alpha A Crystallin/CRYAA (C terminal) conjugated to keyhole limpet haemocyanin.
This product was previously labelled as alpha A Crystallin
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab69552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 20 kDa.|
FunctionMay contribute to the transparency and refractive index of the lens.
Involvement in diseaseDefects in CRYAA are a cause of cataract autosomal dominant (ADC) [MIM:604219]. Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract is the most common treatable cause of visual disability in childhood.
Sequence similaritiesBelongs to the small heat shock protein (HSP20) family.
modificationsO-glycosylated; contains N-acetylglucosamine side chains.
Deamidation of Asn-101 in lens occurs mostly during the first 30 years of age, followed by a small additional amount of deamidation (approximately 5%) during the next approximately 38 years, resulting in a maximum of approximately 50% deamidation during the lifetime of the individual.
Phosphorylation on Ser-122 seems to be developmentally regulated. Absent in the first months of life, it appears during the first 12 years of human lifetime. The relative amount of phosphorylated form versus unphosphorylated form does not change over the lifetime of the individual.
Cellular localizationCytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
- Information by UniProt
- Acry 1 antibody
- Alpha crystallin A chain antibody
- Alpha-crystallin A chain antibody
Anti-alpha A Crystallin/CRYAA antibody (ab69552) at 1/1000 dilution + bovine alpha A Crystallin/CRYAA
Predicted band size: 20 kDa
Observed band size: 20 kDa
All lanes : Anti-alpha A Crystallin/CRYAA antibody (ab69552) at 1/1000 dilution
Lane 1 : Crystalline, alpha native bovine protein
Lane 2 : Crystalline alpha A/CRYAA native bovine protein
Lane 3 : Crytalline alpha B native bovine protein
Lane 4 : Crystalline beta native bovine protein
Predicted band size: 20 kDa
ab69552 has been referenced in 1 publication.
- Su SP et al. Understanding the a-crystallin cell membrane conjunction. Mol Vis 17:2798-807 (2011). WB . PubMed: 22219626