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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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RecombinantRabMAb

Recombinant Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)

  • Datasheet
  • Certificate of Compliance
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Immunocytochemistry/ Immunofluorescence - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)
  • Western blot - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)
  • Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14125(B)] to alpha A Crystallin/CRYAA
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)]
    See all alpha A Crystallin/CRYAA primary antibodies
  • Description

    Rabbit monoclonal [EPR14125(B)] to alpha A Crystallin/CRYAA
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Recombinant human alpha A Crystallin/CRYAA protein (ab48778) can be used as a positive control in WB. Rat and Mouse eyeball. Y79 cells.
  • General notes

     

     

     This product was previously labelled as alpha A Crystallin

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14125(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Tags & Cell Markers
    • Cell Type Markers
    • Other Cell Types
    • Neuroscience
    • Sensory System
    • Visual system

Associated products

  • Alternative Versions

    • Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] - BSA and Azide free (ab250535)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Recombinant human alpha A Crystallin/CRYAA protein (ab48778)
  • Recombinant Protein

    • Recombinant human alpha A Crystallin/CRYAA protein (ab48778)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Rat
Application Abreviews Notes
WB
1/1000 - 1/2000. Predicted molecular weight: 20 kDa.
ICC/IF
1/500.
Notes
WB
1/1000 - 1/2000. Predicted molecular weight: 20 kDa.
ICC/IF
1/500.

Target

  • Function

    May contribute to the transparency and refractive index of the lens.
  • Involvement in disease

    Defects in CRYAA are a cause of cataract autosomal dominant (ADC) [MIM:604219]. Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract is the most common treatable cause of visual disability in childhood.
  • Sequence similarities

    Belongs to the small heat shock protein (HSP20) family.
  • Post-translational
    modifications

    O-glycosylated; contains N-acetylglucosamine side chains.
    Deamidation of Asn-101 in lens occurs mostly during the first 30 years of age, followed by a small additional amount of deamidation (approximately 5%) during the next approximately 38 years, resulting in a maximum of approximately 50% deamidation during the lifetime of the individual.
    Phosphorylation on Ser-122 seems to be developmentally regulated. Absent in the first months of life, it appears during the first 12 years of human lifetime. The relative amount of phosphorylated form versus unphosphorylated form does not change over the lifetime of the individual.
  • Cellular localization

    Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Target information above from: UniProt accession P02489 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1409 Human
    • Entrez Gene: 12954 Mouse
    • Entrez Gene: 24273 Rat
    • Omim: 123580 Human
    • SwissProt: P02489 Human
    • SwissProt: P24622 Mouse
    • SwissProt: P24623 Rat
    • Unigene: 184085 Human
    • Unigene: 1228 Mouse
    • Unigene: 127769 Rat
    see all
  • Alternative names

    • Acry 1 antibody
    • Alpha crystallin A chain antibody
    • Alpha-crystallin A chain antibody
    • CRYA 1 antibody
    • CRYA1 antibody
    • CRYAA antibody
    • CRYAA_HUMAN antibody
    • Crystallin alpha 1 antibody
    • Crystallin alpha A antibody
    • Heat shock protein beta 4 antibody
    • Heat shock protein beta-4 antibody
    • HSPB 4 antibody
    • HspB4 antibody
    • short form antibody
    • Zonular Central Nuclear Cataract antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)

    Immunofluorescence analysis of Y79 cells fixed with acetone labeling alpha A Crystallin/CRYAA with ab181866 at 1/500 dilution followed by Goat anti rabbit IgG(Alexa Fluor®555) at 1/200 dilution and counterstained with Dapi.

  • Western blot - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)
    Western blot - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)
    All lanes : Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866) at 1/2000 dilution

    Lane 1 : Rat eyeball
    Lane 2 : Mouse eyeball

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa

  • Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)
    Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (1)

    Publishing research using ab181866? Please let us know so that we can cite the reference in this datasheet.

    ab181866 has been referenced in 1 publication.

    • Liu X  et al. Long Non-Coding RNA H19 Regulates Human Lens Epithelial Cells Function. Cell Physiol Biochem 50:246-260 (2018). PubMed: 30286462

    Customer reviews and Q&As

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