Overview

  • Product name

    Anti-alpha Actinin 4 antibody
    See all alpha Actinin 4 primary antibodies
  • Description

    Rabbit polyclonal to alpha Actinin 4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Xenopus laevis, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human alpha Actinin 4 (N terminal). The exact sequence is proprietary.
    Database link: O43707

  • Positive control

    • WB: A431, HEK-293T, HeLa, HepG2, NIH/3T3, JC and PC-12 whole cell lysates/extracts. ICC/IF: MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 105 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
  • Tissue specificity

    Widely expressed.
  • Involvement in disease

    Defects in ACTN4 are the cause of focal segmental glomerulosclerosis type 1 (FSGS1) [MIM:603278]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • Sequence similarities

    Belongs to the alpha-actinin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
    Contains 4 spectrin repeats.
  • Cellular localization

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines.
  • Information by UniProt
  • Database links

  • Alternative names

    • actinin 4 antibody
    • Actinin alpha 4 antibody
    • actinin4 antibody
    • ACTN 4 antibody
    • ACTN4 antibody
    • ACTN4_HUMAN antibody
    • Alpha-actinin-4 antibody
    • DKFZp686K23158 antibody
    • F actin cross linking protein antibody
    • F-actin cross-linking protein antibody
    • Focal segmental glomerulosclerosis 1 antibody
    • FSGS 1 antibody
    • FSGS antibody
    • FSGS1 antibody
    • Non muscle alpha actinin 4 antibody
    • Non-muscle alpha-actinin 4 antibody
    see all

Images

  • Anti-alpha Actinin 4 antibody (ab227166) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 105 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-alpha Actinin 4 antibody (ab227166) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 2 : JC whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 105 kDa



    7.5% SDS-PAGE gel.

  • Anti-alpha Actinin 4 antibody (ab227166) at 1/1000 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg

    Predicted band size: 105 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-alpha Actinin 4 antibody (ab227166) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 105 kDa



    7.5% SDS-PAGE gel.

  • Ice-cold methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for alpha Actinin 4 (green) using ab227166 at 1/500 dilution in ICC/IF.

    Nuclear Counterstain: Hoechst 33342.

References

ab227166 has not yet been referenced specifically in any publications.

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