Anti-alpha Actinin 4 antibody - C-terminal (ab137564)
Key features and details
- Rabbit polyclonal to alpha Actinin 4 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-alpha Actinin 4 antibody - C-terminal
See all alpha Actinin 4 primary antibodies -
Description
Rabbit polyclonal to alpha Actinin 4 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within C terminal amino acids 698-911 of Human alpha Actinin 4
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Positive control
- HepG2, NIH 3T3, Mouse brain or PC12 whole cell lysate
Paraformaldehyde fixed HeLa cells
A549 xenograft
- HepG2, NIH 3T3, Mouse brain or PC12 whole cell lysate
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General notes
Keep as concentrated solution.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- HepG2 cytoplasmic extract lysate (ab14659)
- HepG2 nuclear extract lysate (ab14660)
- NIH 3T3 cytoplasmic extract lysate (ab14873)
- NIH 3T3 nuclear extract lysate (ab14874)
- PC12 cytoplasmic extract lysate (ab14883)
- PC12 nuclear extract lysate (ab14884)
- Mouse brain tissue lysate - total protein (ab30151)
- NIH 3T3 whole cell lysate (ab7179)
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab137564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 105 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. -
Tissue specificity
Widely expressed. -
Involvement in disease
Defects in ACTN4 are the cause of focal segmental glomerulosclerosis type 1 (FSGS1) [MIM:603278]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. -
Sequence similarities
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
Cellular localization
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines. - Information by UniProt
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Database links
- Entrez Gene: 81 Human
- Entrez Gene: 60595 Mouse
- Entrez Gene: 63836 Rat
- Omim: 604638 Human
- SwissProt: O43707 Human
- SwissProt: P57780 Mouse
- SwissProt: Q9QXQ0 Rat
- Unigene: 270291 Human
see all -
Alternative names
- actinin 4 antibody
- Actinin alpha 4 antibody
- actinin4 antibody
see all
Images
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Western blot - Anti-alpha Actinin 4 antibody - C-terminal (ab137564)Anti-alpha Actinin 4 antibody - C-terminal (ab137564) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 105 kDa
7.5% SDS PAGE -
Western blot - Anti-alpha Actinin 4 antibody - C-terminal (ab137564)Anti-alpha Actinin 4 antibody - C-terminal (ab137564) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 105 kDa
7.5% SDS PAGE -
Western blot - Anti-alpha Actinin 4 antibody - C-terminal (ab137564)Anti-alpha Actinin 4 antibody - C-terminal (ab137564) at 1/1000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 105 kDa
5% SDS PAGE -
Western blot - Anti-alpha Actinin 4 antibody - C-terminal (ab137564)Anti-alpha Actinin 4 antibody - C-terminal (ab137564) at 1/1000 dilution + PC12 whole cell lysate at 30 µg
Predicted band size: 105 kDa
7.5 % SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-alpha Actinin 4 antibody - C-terminal (ab137564)Confocal immunofluorescence analysis of paraformaldehyde fixed HeLa cells, labelling alpha Actinin 4 with ab137564 (Green) at 1/500 dilution. Alpha tubulin filaments were labelled in Red. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin 4 antibody - C-terminal (ab137564)Immunohistochemical analysis of paraffin embedded A549 xenograft, labelling alpha Actinin 4 with ab137564 at 1/500 dilution.
Protocols
References (1)
ab137564 has been referenced in 1 publication.
- Willms E et al. Cells release subpopulations of exosomes with distinct molecular and biological properties. Sci Rep 6:22519 (2016). WB . PubMed: 26931825
