Anti-alpha Actinin 4 antibody - C-terminal (ab137564)
- Datasheet
- References (1)
- Protocols
Overview
-
Product name
Anti-alpha Actinin 4 antibody - C-terminal
See all alpha Actinin 4 primary antibodies -
Description
Rabbit polyclonal to alpha Actinin 4 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within C terminal amino acids 698-911 of Human alpha Actinin 4
-
Positive control
- HepG2, NIH 3T3, Mouse brain or PC12 whole cell lysate
Paraformaldehyde fixed HeLa cells
A549 xenograft
- HepG2, NIH 3T3, Mouse brain or PC12 whole cell lysate
-
General notes
Keep as concentrated solution.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
- HepG2 cytoplasmic extract lysate (ab14659)
- HepG2 nuclear extract lysate (ab14660)
- NIH 3T3 cytoplasmic extract lysate (ab14873)
- NIH 3T3 nuclear extract lysate (ab14874)
- PC12 cytoplasmic extract lysate (ab14883)
- PC12 nuclear extract lysate (ab14884)
- Mouse brain tissue lysate - total protein (ab30151)
- NIH 3T3 whole cell lysate (ab7179)
- HepG2 whole cell lysate (ab7900)
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab137564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 105 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). | |
| ICC/IF | 1/100 - 1/1000. |
Target
-
Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. -
Tissue specificity
Widely expressed. -
Involvement in disease
Defects in ACTN4 are the cause of focal segmental glomerulosclerosis type 1 (FSGS1) [MIM:603278]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. -
Sequence similarities
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
Cellular localization
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines. - Information by UniProt
-
Database links
- Entrez Gene: 81 Human
- Entrez Gene: 60595 Mouse
- Entrez Gene: 63836 Rat
- Omim: 604638 Human
- SwissProt: O43707 Human
- SwissProt: P57780 Mouse
- SwissProt: Q9QXQ0 Rat
- Unigene: 270291 Human
see all -
Alternative names
- actinin 4 antibody
- Actinin alpha 4 antibody
- actinin4 antibody
see all
Images
-
Western blot - Anti-alpha Actinin 4 antibody - C-terminal (ab137564)Anti-alpha Actinin 4 antibody - C-terminal (ab137564) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 105 kDa
7.5% SDS PAGE -
Western blot - Anti-alpha Actinin 4 antibody - C-terminal (ab137564)Anti-alpha Actinin 4 antibody - C-terminal (ab137564) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 105 kDa
7.5% SDS PAGE -
Western blot - Anti-alpha Actinin 4 antibody - C-terminal (ab137564)Anti-alpha Actinin 4 antibody - C-terminal (ab137564) at 1/1000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 105 kDa
5% SDS PAGE -
Western blot - Anti-alpha Actinin 4 antibody - C-terminal (ab137564)Anti-alpha Actinin 4 antibody - C-terminal (ab137564) at 1/1000 dilution + PC12 whole cell lysate at 30 µg
Predicted band size: 105 kDa
7.5 % SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-alpha Actinin 4 antibody - C-terminal (ab137564)Confocal immunofluorescence analysis of paraformaldehyde fixed HeLa cells, labelling alpha Actinin 4 with ab137564 (Green) at 1/500 dilution. Alpha tubulin filaments were labelled in Red. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin 4 antibody - C-terminal (ab137564)Immunohistochemical analysis of paraffin embedded A549 xenograft, labelling alpha Actinin 4 with ab137564 at 1/500 dilution.
Protocols
References
This product has been referenced in:
- Willms E et al. Cells release subpopulations of exosomes with distinct molecular and biological properties. Sci Rep 6:22519 (2016). WB . Read more (PubMed: 26931825) »
