Product nameAnti-alpha Actinin 4 antibody [EPR2533(2)] (HRP)
See all alpha Actinin 4 primary antibodies
DescriptionRabbit monoclonal [EPR2533(2)] to alpha Actinin 4 (HRP)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human alpha Actinin 4 aa 1-100 (N terminal). The exact sequence is proprietary.
- WB: A431, MCF7 and HeLa whole cell lysates. IHC/P: normal human adrenal gland tissue section.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-alpha Actinin 4 antibody [EPR2533(2)] (ab108198)
- Anti-alpha Actinin 4 antibody [EPR2533(2)] (Alexa Fluor® 488) (ab198608)
- Anti-alpha Actinin 4 antibody [EPR2533(2)] (Alexa Fluor® 647) (ab198610)
- Anti-alpha Actinin 4 antibody [EPR2533(2)] - BSA and Azide free (ab204919)
- Anti-alpha Actinin 4 antibody [EPR2533(2)] (Phycoerythrin) (ab223936)
Our Abpromise guarantee covers the use of ab199072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/5000. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).|
FunctionF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Tissue specificityWidely expressed.
Involvement in diseaseDefects in ACTN4 are the cause of focal segmental glomerulosclerosis type 1 (FSGS1) [MIM:603278]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
Sequence similaritiesBelongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats.
Cellular localizationNucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines.
- Information by UniProt
- actinin 4 antibody
- Actinin alpha 4 antibody
- actinin4 antibody
All lanes : Anti-alpha Actinin 4 antibody [EPR2533(2)] (HRP) (ab199072) at 1/5000 dilution
Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 :
HeLa whole cell lysate (ab150035)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 105 kDa
Observed band size: 105 kDa
Exposure time: 20 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199072 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
IHC image of alpha Actinin 4 staining in a section of formalin-fixed paraffin-embedded normal human adrenal gland tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab199072 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ab199072 has not yet been referenced specifically in any publications.