Recombinant Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)
![Western blot - Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)](https://www.abcam.com/ps/products/108/ab108201/Images/ab108201-318641-ab108201-KO-WB-DWAR.jpg "Western blot - Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4410] to alpha Actinin 4
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-alpha Actinin 4 antibody [EPR4410]
See all alpha Actinin 4 primary antibodies -
Description
Rabbit monoclonal [EPR4410] to alpha Actinin 4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, MCF-7, C2C12, A673, and L6 cell lysates; Human kidney tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4410 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 105 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 105 kDa). |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. -
Tissue specificity
Widely expressed. -
Involvement in disease
Defects in ACTN4 are the cause of focal segmental glomerulosclerosis type 1 (FSGS1) [MIM:603278]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. -
Sequence similarities
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
Cellular localization
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines. - Information by UniProt
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Database links
- Entrez Gene: 81 Human
- Entrez Gene: 60595 Mouse
- Entrez Gene: 63836 Rat
- Omim: 604638 Human
- SwissProt: O43707 Human
- SwissProt: P57780 Mouse
- SwissProt: Q9QXQ0 Rat
- Unigene: 270291 Human
see all -
Alternative names
- actinin 4 antibody
- Actinin alpha 4 antibody
- actinin4 antibody
see all
Images
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Western blot - Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)All lanes : Anti-alpha Actinin 4 antibody [EPR4410] (ab108201) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate at 40 µg
Lane 2 : ACTN4 (alpha Actinin 4) knockout HAP1 whole cell lysate at 40 µg
Lane 3 : HeLa whole cell lysate at 20 µg
Predicted band size: 105 kDa
Observed band size: 105 kDaLanes 1 - 3: Merged signal (red and green). Green - ab108201 observed at 105 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab108201 was shown to recognize alpha Actinin 4 in wild-type HAP1 cells as signal was lost at the expected MW in ACTN4 (alpha Actinin 4) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ACTN4 (alpha Actinin 4) knockout samples were subjected to SDS-PAGE. Ab108201 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)](https://www.abcam.com/ps/products/108/ab108201/Images/alpha-Actinin-4-Primary-antibodies-ab108201-1.jpg)
Western blot - Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)All lanes : Anti-alpha Actinin 4 antibody [EPR4410] (ab108201) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : C2C12 cell lysate
Lane 4 : A673 cell lysate
Lane 5 : L6 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 105 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)](https://www.abcam.com/ps/products/108/ab108201/Images/alpha-Actinin-4-Primary-antibodies-ab108201-2.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)ab108201, at 1/100 dilution, staining alpha Actinin 4 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)](https://www.abcam.com/ps/products/108/ab108201/Images/ab108201-4-benefits-of-recombinant-antibodies.png)
Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab108201 has not yet been referenced specifically in any publications.
