Recombinant
RabMAb

Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)

Submit an Abreview Submit a Question

Overview

  • Product name
    Anti-alpha Actinin 4 antibody [EPR4410]
    See all alpha Actinin 4 primary antibodies
  • Description
    Rabbit monoclonal [EPR4410] to alpha Actinin 4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human alpha Actinin 4.

  • Positive control
    • HeLa, MCF-7, C2C12, A673, and L6 cell lysates; Human kidney tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 105 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.

    • Target

    • Function
      F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
    • Tissue specificity
      Widely expressed.
    • Involvement in disease
      Defects in ACTN4 are the cause of focal segmental glomerulosclerosis type 1 (FSGS1) [MIM:603278]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
    • Sequence similarities
      Belongs to the alpha-actinin family.
      Contains 1 actin-binding domain.
      Contains 2 CH (calponin-homology) domains.
      Contains 2 EF-hand domains.
      Contains 4 spectrin repeats.
    • Cellular localization
      Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • actinin 4 antibody
      • Actinin alpha 4 antibody
      • actinin4 antibody
      • ACTN 4 antibody
      • ACTN4 antibody
      • ACTN4_HUMAN antibody
      • Alpha-actinin-4 antibody
      • DKFZp686K23158 antibody
      • F actin cross linking protein antibody
      • F-actin cross-linking protein antibody
      • Focal segmental glomerulosclerosis 1 antibody
      • FSGS 1 antibody
      • FSGS antibody
      • FSGS1 antibody
      • Non muscle alpha actinin 4 antibody
      • Non-muscle alpha-actinin 4 antibody
      see all

    Images

    • Western blot - Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)
      Western blot - Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)
      All lanes : Anti-alpha Actinin 4 antibody [EPR4410] (ab108201) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF-7 cell lysate
      Lane 3 : C2C12 cell lysate
      Lane 4 : A673 cell lysate
      Lane 5 : L6 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 105 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)
      ab108201, at 1/100 dilution, staining alpha Actinin 4 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    References

    ab108201 has not yet been referenced specifically in any publications.

    Publishing research using ab108201? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab108201.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up