Product nameAnti-alpha Actinin antibody [AT6/172]
See all alpha Actinin primary antibodies
DescriptionMouse monoclonal [AT6/172] to alpha Actinin
SpecificityRecognizes and is specific for human alpha Actinin 1 isoform (non-muscle) at 100 kDa. This antibody may recognize other isoforms of alpha-actinin (1-4). There is no specific epitope information available. The clone number has been updated from (0.T.02) to (AT6/172) both clone numbers name the same antibody clone.
Tested applicationsSuitable for: WB, IHC-Fr, IHC (Methanol fixed), IHC-P, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Sheep, Rabbit, Human
Full length protein corresponding to Human alpha Actinin.
Database link: P12814
- WB: A431 whole cell lysate (ab7909), Human pancreatic cancer whole cell lysate. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.6
Preservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab18061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 103 kDa.|
|IHC-Fr||Use a concentration of 15 µg/ml.|
|IHC (Methanol fixed)||Use a concentration of 15 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 15 µg/ml.|
FunctionF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Sequence similaritiesBelongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.
- Information by UniProt
- actinin 1 smooth muscle antibody
- Actinin alpha 1 antibody
- actinin, alpha 1 antibody
Anti-alpha Actinin antibody [AT6/172] (ab18061) at 1/2000 dilution + Human pancreatic cancer cell line - whole cell lysate at 20 µg
HRP conjugated goat anti-mouse antibody at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 103 kDa
Observed band size: 103 kDa
Exposure time: 1 minute
ICC/IF image of ab18061 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed in 4% formaldehyde for 10 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3 M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18061, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
ab18061 staining alpha Actinin in Mouse skin tumor tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated Donkey anti-mouse polyclonal (1/400) was used as the secondary antibody.
This product has been referenced in:
- Zheng C et al. Gastrodin Inhibits Store-Operated Ca(2+) Entry and Alleviates Cardiac Hypertrophy. Front Pharmacol 8:222 (2017). ICC/IF ; Mouse . Read more (PubMed: 28487655) »
- Han X et al. Zyxin regulates endothelial von Willebrand factor secretion by reorganizing actin filaments around exocytic granules. Nat Commun 8:14639 (2017). Read more (PubMed: 28256511) »