Key features and details
- Rabbit polyclonal to alpha Actinin/ACTN1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-alpha Actinin/ACTN1 antibody
See all alpha Actinin/ACTN1 primary antibodies
DescriptionRabbit polyclonal to alpha Actinin/ACTN1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
Recombinant fragment corresponding to Human alpha Actinin/ACTN1 aa 220-380.
Database link: P12814
- 293T, A431, H1299, HeLa S3, HepG2, MOLT4, NIH 3T3 and Raji whole cell lysates. Human SW480 xenograft tissue.
General notesKeep as concentrated solution.
This product was previously labelled as alpha Actinin
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 103 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).|
FunctionF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Sequence similaritiesBelongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.
- Information by UniProt
- actinin 1 smooth muscle antibody
- Actinin alpha 1 antibody
- actinin, alpha 1 antibody
Anti-alpha Actinin/ACTN1 antibody (ab155480) at 1/1000 dilution + mouse NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 103 kDa
7.5% SDS PAGE
All lanes : Anti-alpha Actinin/ACTN1 antibody (ab155480) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLa S3 whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lane 5 : MOLT4 whole cell lysate
Lane 6 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 103 kDa
7.5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft tissue, labeling alpha Actinin/ACTN1 using ab155480 at a 1/100 dilution.
ab155480 has not yet been referenced specifically in any publications.