Overview

  • Product name

    Anti-alpha Actinin/ACTN1 antibody
    See all alpha Actinin/ACTN1 primary antibodies
  • Description

    Rabbit polyclonal to alpha Actinin/ACTN1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human alpha Actinin/ACTN1 aa 220-380.
    Database link: P12814

  • Positive control

    • 293T, A431, H1299, HeLa S3, HepG2, MOLT4, NIH 3T3 and Raji whole cell lysates. Human SW480 xenograft tissue.
  • General notes

    Keep as concentrated solution.

     This product was previously labelled as alpha Actinin

     

Properties

Applications

Our Abpromise guarantee covers the use of ab155480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 103 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Target

Images

  • Anti-alpha Actinin/ACTN1 antibody (ab155480) at 1/1000 dilution + mouse NIH 3T3 whole cell lysate at 30 µg

    Predicted band size: 103 kDa



    7.5% SDS PAGE
  • All lanes : Anti-alpha Actinin/ACTN1 antibody (ab155480) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HeLa S3 whole cell lysate
    Lane 4 : HepG2 whole cell lysate
    Lane 5 : MOLT4 whole cell lysate
    Lane 6 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 103 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft tissue, labeling alpha Actinin/ACTN1 using ab155480 at a 1/100 dilution.

References

ab155480 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155480.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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