Validated using a knockout cell line

Recombinant Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - BSA and Azide free (ab185204)


  • Product name

    Anti-alpha Actinin/ACTN1 antibody [EP2528Y] - BSA and Azide free
    See all alpha Actinin/ACTN1 primary antibodies
  • Description

    Rabbit monoclonal [EP2528Y] to alpha Actinin/ACTN1 - BSA and Azide free
  • Host species

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human alpha Actinin/ACTN1 aa 750-850 (C terminal). The exact sequence is proprietary.

  • Positive control

    • NIH 3T3 cell, human skeletal muscle tissue and human heart tissue lysates.
  • General notes

    ab185204 is the carrier-free version of ab81265 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.


    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab185204 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as alpha Actinin

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab185204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
    • Sequence similarities

      Belongs to the alpha-actinin family.
      Contains 1 actin-binding domain.
      Contains 2 CH (calponin-homology) domains.
      Contains 2 EF-hand domains.
      Contains 4 spectrin repeats.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.
    • Information by UniProt
    • Database links

    • Alternative names

      • actinin 1 smooth muscle antibody
      • Actinin alpha 1 antibody
      • actinin, alpha 1 antibody
      • ACTN 1 antibody
      • Actn1 antibody
      • ACTN1_HUMAN antibody
      • Alpha Actinin 1 antibody
      • Alpha actinin cytoskeletal isoform antibody
      • Alpha-actinin cytoskeletal isoform antibody
      • Alpha-actinin-1 antibody
      • BDPLT15 antibody
      • F actin cross linking protein antibody
      • F-actin cross-linking protein antibody
      • FLJ40884 antibody
      • FLJ54432 antibody
      • Non muscle alpha actinin 1 antibody
      • Non-muscle alpha-actinin-1 antibody
      see all


    ab185204 has not yet been referenced specifically in any publications.

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