Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421)
Key features and details
- Mouse monoclonal [OTI7A4] to alpha Actinin/ACTN1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG2a
Overview
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Product name
Anti-alpha Actinin/ACTN1 antibody [OTI7A4]
See all alpha Actinin/ACTN1 primary antibodies -
Description
Mouse monoclonal [OTI7A4] to alpha Actinin/ACTN1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, African green monkey -
Immunogen
Recombinant fragment corresponding to Human alpha Actinin/ACTN1 aa 650-893. Protein produced in E.coli.
Database link: NP_001093 -
Positive control
- ICC/IF: HeLa cells transiently transfected by pCMV6-ENTRY alpha Actinin/ATCN1. HeLa cells. WB: HEK-293T cell transient overexpression lysate, HepG, NIH/3T3, HeLa, SVT2, A549, COS-7, Jurkat, PC-12, and MCF7 cell extracts. Mouse spleen, brain, and liver tissue extracts. IHC-P: Human breast, kidney, liver, ovary and bladder tissue.
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General notes
The clone number has been updated from 7A4 to OTI7A4, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI7A4 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Hep G2 whole cell lysate (ab166833)
- Mouse spleen tissue lysate - total protein (ab29293)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- NIH/3T3 whole cell lysate (ab7179)
- Jurkat whole cell lysate (ab7899)
- Mouse spleen tissue lysate - total protein (ab7937)
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab90421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/200 - 1/2000. Predicted molecular weight: 103 kDa.
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ICC/IF |
1/100.
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IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/200 - 1/2000. Predicted molecular weight: 103 kDa. |
ICC/IF
1/100. |
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. -
Sequence similarities
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. - Information by UniProt
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Database links
- Entrez Gene: 87 Human
- Entrez Gene: 109711 Mouse
- Entrez Gene: 81634 Rat
- Omim: 102575 Human
- SwissProt: P12814 Human
- SwissProt: Q7TPR4 Mouse
- SwissProt: Q9Z1P2 Rat
- Unigene: 509765 Human
see all -
Alternative names
- actinin 1 smooth muscle antibody
- Actinin alpha 1 antibody
- actinin, alpha 1 antibody
see all
Images
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All lanes : Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421) at 1/200 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) extracts
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) extracts
Lane 3 : SVT2 (Mouse cell line) extracts
Lane 4 : A549 (Human lung carcinoma cell line) extracts
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) extracts
Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) extracts
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) extracts
Lane 8 : MCF7 (Human breast adenocarcinoma cell line) extracts
Lysates/proteins at 10 µg per lane.
Predicted band size: 103 kDa -
Paraffin-embedded human breast tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for alpha Actinin/ATCN1 using ab90421 at a 1/100 dilution in ICC/IF.
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Paraffin-embedded human kidney tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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Paraffin-embedded human bladder tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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Paraffin-embedded human ovary tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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Paraffin-embedded human liver tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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pCMV6-ENTRY alpha Actinin/ATCN1 cDNA transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for alpha Actinin/ATCN1 using ab90421 at a 1/100 dilution in ICC/IF.
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All lanes : Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421) at 1/200 dilution
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) extracts
Lane 2 : Mouse brain tissue extracts
Lane 3 : Mouse liver tissue extracts
Lane 4 : Mouse spleen tissue extracts
Lysates/proteins at 10 µg per lane.
Predicted band size: 103 kDa -
All lanes : Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were transfected with the pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells were transfected with pCMV6-ENTRY alpha Actinin/ATCN1
cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 103 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab90421 has not yet been referenced specifically in any publications.