Overview

  • Product name

    Anti-alpha Actinin/ACTN1 antibody [OTI7A4]
    See all alpha Actinin/ACTN1 primary antibodies
  • Description

    Mouse monoclonal [OTI7A4] to alpha Actinin/ACTN1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, African green monkey
  • Immunogen

    Recombinant fragment corresponding to Human alpha Actinin/ACTN1 aa 650-893. Protein produced in E.coli.
    Database link: NP_001093

  • Positive control

    • ICC/IF: HeLa cells transiently transfected by pCMV6-ENTRY alpha Actinin/ATCN1. HeLa cells. WB: HEK-293T cell transient overexpression lysate, HepG, NIH/3T3, HeLa, SVT2, A549, COS-7, Jurkat, PC-12, and MCF7 cell extracts. Mouse spleen tissue extracts. IHC-P: Human breast, kidney, liver, ovary and bladder tissue.
  • General notes

    The clone number has been updated from 7A4 to OTI7A4, both clone numbers name the same clone.

     This product was previously labelled as alpha Actinin

     

Properties

Applications

Our Abpromise guarantee covers the use of ab90421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Predicted molecular weight: 103 kDa.
ICC/IF 1/100.
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
  • Sequence similarities

    Belongs to the alpha-actinin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
    Contains 4 spectrin repeats.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.
  • Information by UniProt
  • Database links

  • Alternative names

    • actinin 1 smooth muscle antibody
    • Actinin alpha 1 antibody
    • actinin, alpha 1 antibody
    • ACTN 1 antibody
    • Actn1 antibody
    • ACTN1_HUMAN antibody
    • Alpha Actinin 1 antibody
    • Alpha actinin cytoskeletal isoform antibody
    • Alpha-actinin cytoskeletal isoform antibody
    • Alpha-actinin-1 antibody
    • BDPLT15 antibody
    • F actin cross linking protein antibody
    • F-actin cross-linking protein antibody
    • FLJ40884 antibody
    • FLJ54432 antibody
    • Non muscle alpha actinin 1 antibody
    • Non-muscle alpha-actinin-1 antibody
    see all

Images

  • All lanes : Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421) at 1/200 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) extracts
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) extracts
    Lane 3 : SVT2 (Mouse cell line) extracts
    Lane 4 : A549 (Human lung carcinoma cell line) extracts
    Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) extracts
    Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) extracts
    Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) extracts
    Lane 8 : MCF7 (Human breast adenocarcinoma cell line) extracts

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 103 kDa

  • Paraffin-embedded human breast tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for alpha Actinin/ATCN1 using ab90421 at a 1/100 dilution in ICC/IF.

  • Paraffin-embedded human kidney tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Paraffin-embedded human bladder tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Paraffin-embedded human ovary tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Paraffin-embedded human liver tissue stained for alpha Actinin/ATCN1 with ab90421 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • pCMV6-ENTRY alpha Actinin/ATCN1 cDNA transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for alpha Actinin/ATCN1 using ab90421 at a 1/100 dilution in ICC/IF.

  • All lanes : Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421) at 1/200 dilution

    Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) extracts
    Lane 2 : Mouse brain tissue extracts
    Lane 3 : Mouse liver tissue extracts
    Lane 4 : Mouse spleen tissue extracts

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 103 kDa

  • All lanes : Anti-alpha Actinin/ACTN1 antibody [OTI7A4] (ab90421) at 1/2000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were transfected with the pCMV6-ENTRY control cDNA
    Lane 2 : HEK-293T cells were transfected with pCMV6-ENTRY alpha Actinin/ATCN1
    cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 103 kDa

References

ab90421 has not yet been referenced specifically in any publications.

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