Anti-alpha Adducin antibody (ab151471)
Key features and details
- Rabbit polyclonal to alpha Adducin
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-alpha Adducin antibody
See all alpha Adducin primary antibodies -
Description
Rabbit polyclonal to alpha Adducin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment corresponding to a region within amino acids 107-425 of Human alpha Adducin (P35611).
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Positive control
- 293T, A431, Jurakt and Raji whole cell lysates; A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
Chicken
Cow
Xenopus laevis
Zebrafish
Xenopus tropicalis
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Detects a band of approximately 110 kDa (predicted molecular weight: 70-84 kDa).
|
|
| ICC/IF |
1/100 - 1/1000.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Detects a band of approximately 110 kDa (predicted molecular weight: 70-84 kDa). |
|
ICC/IF
1/100 - 1/1000. |
Target
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Function
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. -
Tissue specificity
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit. -
Sequence similarities
Belongs to the aldolase class II family. Adducin subfamily. -
Domain
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region. -
Cellular localization
Cytoplasm > cytoskeleton. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 118 Human
- Entrez Gene: 11518 Mouse
- Entrez Gene: 24170 Rat
- Entrez Gene: 555511 Zebrafish
- Omim: 102680 Human
- SwissProt: P35611 Human
- SwissProt: Q9QYC0 Mouse
- SwissProt: Q63028 Rat
see all -
Alternative names
- ADD A antibody
- ADD1 antibody
- ADDA antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin antibody (ab151471)Immunofluorescence analysis of methanol-fixed A431 cells labeling alpha Adducin with ab151471 at 1/500 dilution (green). Alpha-tubulin filaments were labeled with an anti-alpha tubulin monoclonal antibody at 1/2000 dilution (red).
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Western blot - Anti-alpha Adducin antibody (ab151471)Anti-alpha Adducin antibody (ab151471) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 70-84 kDa
7.5% SDS PAGE
Protocols
References (0)
ab151471 has not yet been referenced specifically in any publications.
