Anti-alpha Adducin antibody (ab151471)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-alpha Adducin antibody
See all alpha Adducin primary antibodies -
Description
Rabbit polyclonal to alpha Adducin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to a region within amino acids 107-425 of Human alpha Adducin (P35611).
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Positive control
- 293T, A431, Jurakt and Raji whole cell lysates; A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 1% BSA, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab151471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Detects a band of approximately 110 kDa (predicted molecular weight: 70-84 kDa). | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. -
Tissue specificity
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit. -
Sequence similarities
Belongs to the aldolase class II family. Adducin subfamily. -
Domain
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region. -
Cellular localization
Cytoplasm > cytoskeleton. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 118 Human
- Entrez Gene: 11518 Mouse
- Entrez Gene: 24170 Rat
- Entrez Gene: 555511 Zebrafish
- Omim: 102680 Human
- SwissProt: P35611 Human
- SwissProt: Q9QYC0 Mouse
- SwissProt: Q63028 Rat
see all -
Alternative names
- ADD A antibody
- ADD1 antibody
- ADDA antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin antibody (ab151471)Immunofluorescence analysis of methanol-fixed A431 cells labeling alpha Adducin with ab151471 at 1/500 dilution (green). Alpha-tubulin filaments were labeled with an anti-alpha tubulin monoclonal antibody at 1/2000 dilution (red). -
Western blot - Anti-alpha Adducin antibody (ab151471)Anti-alpha Adducin antibody (ab151471) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 70-84 kDa
7.5% SDS PAGE
References
ab151471 has not yet been referenced specifically in any publications.
