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    alpha-adducin-antibody-ab151471.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin
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Anti-alpha Adducin antibody (ab151471)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin antibody (ab151471)
  • Western blot - Anti-alpha Adducin antibody (ab151471)

Key features and details

  • Rabbit polyclonal to alpha Adducin
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-alpha Adducin antibody
    See all alpha Adducin primary antibodies
  • Description

    Rabbit polyclonal to alpha Adducin
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 107-425 of Human alpha Adducin (P35611).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • 293T, A431, Jurakt and Raji whole cell lysates; A431 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKC
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKA
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calmodulin Pathway

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • A-431 whole cell lysate (ab30132)
    • Jurkat whole cell lysate (ab7899)
    • A-431 whole cell lysate (ab7909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Mouse
Rat
Chicken
Cow
Xenopus laevis
Zebrafish
Xenopus tropicalis
Application Abreviews Notes
WB
1/500 - 1/3000. Detects a band of approximately 110 kDa (predicted molecular weight: 70-84 kDa).
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Detects a band of approximately 110 kDa (predicted molecular weight: 70-84 kDa).
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
  • Tissue specificity

    Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
  • Sequence similarities

    Belongs to the aldolase class II family. Adducin subfamily.
  • Domain

    Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell membrane.
  • Target information above from: UniProt accession P35611 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 118 Human
    • Entrez Gene: 11518 Mouse
    • Entrez Gene: 24170 Rat
    • Entrez Gene: 555511 Zebrafish
    • Omim: 102680 Human
    • SwissProt: P35611 Human
    • SwissProt: Q9QYC0 Mouse
    • SwissProt: Q63028 Rat
    • Unigene: 183706 Human
    • Unigene: 289106 Mouse
    • Unigene: 5788 Rat
    see all
  • Alternative names

    • ADD A antibody
    • ADD1 antibody
    • ADDA antibody
    • ADDA_HUMAN antibody
    • Adducin 1 (alpha) antibody
    • Alpha adducin antibody
    • Alpha-adducin antibody
    • Erythrocyte adducin alpha subunit antibody
    • Erythrocyte adducin subunit alpha antibody
    • MGC3339 antibody
    • MGC44427 antibody
    • OTTHUMP00000151163 antibody
    • OTTHUMP00000151164 antibody
    • OTTHUMP00000151165 antibody
    • OTTHUMP00000218664 antibody
    • OTTHUMP00000218665 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin antibody (ab151471)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin antibody (ab151471)
    Immunofluorescence analysis of methanol-fixed A431 cells labeling alpha Adducin with ab151471 at 1/500 dilution (green). Alpha-tubulin filaments were labeled with an anti-alpha tubulin monoclonal antibody at 1/2000 dilution (red).
  • Western blot - Anti-alpha Adducin antibody (ab151471)
    Western blot - Anti-alpha Adducin antibody (ab151471)
    Anti-alpha Adducin antibody (ab151471) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 70-84 kDa



    7.5% SDS PAGE

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab151471? Please let us know so that we can cite the reference in this datasheet.

    ab151471 has not yet been referenced specifically in any publications.

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