Product nameAnti-alpha Adducin antibody
See all alpha Adducin primary antibodies
DescriptionRabbit polyclonal to alpha Adducin
SpecificityThere are 2 isoforms of alpha adducin on the Uniprot protein database page for mouse. The antibody immunogen has high alignment to isoform 1: Isoform 1 80 kDa 100% Isoform 2 69 kDa 30% The aduccin protein is made up of 4 subunits, including the alpha beta and gamma subunits (according to the Uniprot page), Alignment of the immunogen with the mouse beta adducin: http://www.uniprot.org/uniprot/Q9QYB8 isoform 1 100% isoform 2 30% isoform 3 100% And with the mouse gamma adducin: http://www.uniprot.org/uniprot/Q9QYB5 isoform 1 92% Isoform 2 92% The antibody has not been specifically tested for detection of the other subunits, however according to the high alignments, the antibody is likely to detect several isoforms of these beta and gamma subunits.
Tested applicationsSuitable for: IHC-P, WB, ELISA, ICC/IF, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthesized non-phosphopeptide derived from human alpha Adducin around the phosphorylation site of serine 726 (T-P-SP-F-L).
- Human brain tissue or extracts from Hela cells treated with Forskolin.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab51130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|IP||Use at an assay dependent concentration. PubMed: 22039052|
FunctionMembrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Tissue specificityExpressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
Sequence similaritiesBelongs to the aldolase class II family. Adducin subfamily.
DomainEach subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
Cellular localizationCytoplasm > cytoskeleton. Cell membrane.
- Information by UniProt
- ADD A antibody
- ADD1 antibody
- ADDA antibody
All lanes : Anti-alpha Adducin antibody (ab51130)
Lane 1 : Extracts from Hela cells treated
with Forskolin (40nM, 30min) with no peptide
Lane 2 : Extracts from Hela cells treated
with Forskolin (40nM, 30min) with with immunising peptide
Predicted band size: 81 kDa
This image shows paraffin-embedded human brain tissue stained with ab51130 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue
ICC/IF image of ab51130 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51130, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab51130 used in Immunoprecipitation and Western Blot.
Immunoprecipitation of NLN (EC 18.104.22.168), Thimet Oligopeptidase (thimet OP; EC 22.214.171.124), a-Adducin, and collapsin response mediator protein 2 (CRMP2) from photoradiolabeled P10 mouse forebrain membrane preparations. Immunoprecipitation of each protein was carried out from parallel nonspecific (NS) and total (Tot) photoradiolabeled groups.
a, equal amounts of NS and total sample of each protein immunoprecipitate were used to count the radioactive signal. Bck, background radioactivity; ***, p < 0.001 versus all other groups; n = 3–4).
b–e, representative Western blot confirmation of successful immunoprecipitation of each candidate protein from NS and total samples.
This product has been referenced in:
- Hauser M et al. The Spectrin-Actin-Based Periodic Cytoskeleton as a Conserved Nanoscale Scaffold and Ruler of the Neural Stem Cell Lineage. Cell Rep 24:1512-1522 (2018). Read more (PubMed: 30089262) »
- Han B et al. Structural organization of the actin-spectrin-based membrane skeleton in dendrites and soma of neurons. Proc Natl Acad Sci U S A 114:E6678-E6685 (2017). Read more (PubMed: 28739933) »