• Product name
    Anti-alpha Adducin antibody
    See all alpha Adducin primary antibodies
  • Description
    Rabbit polyclonal to alpha Adducin
  • Host species
  • Specificity
    There are 2 isoforms of alpha adducin on the Uniprot protein database page for mouse. The antibody immunogen has high alignment to isoform 1: Isoform 1 80 kDa 100% Isoform 2 69 kDa 30% The aduccin protein is made up of 4 subunits, including the alpha beta and gamma subunits (according to the Uniprot page), Alignment of the immunogen with the mouse beta adducin: http://www.uniprot.org/uniprot/Q9QYB8 isoform 1 100% isoform 2 30% isoform 3 100% And with the mouse gamma adducin: http://www.uniprot.org/uniprot/Q9QYB5 isoform 1 92% Isoform 2 92% The antibody has not been specifically tested for detection of the other subunits, however according to the high alignments, the antibody is likely to detect several isoforms of these beta and gamma subunits.
  • Tested applications
    Suitable for: IHC-P, WB, ELISA, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized non-phosphopeptide derived from human alpha Adducin around the phosphorylation site of serine 726 (T-P-SP-F-L).

  • Positive control
    • Human brain tissue or extracts from Hela cells treated with Forskolin.



Our Abpromise guarantee covers the use of ab51130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
ELISA 1/40000.
ICC/IF Use a concentration of 1 - 5 µg/ml.
IP Use at an assay dependent concentration. PubMed: 22039052


  • Function
    Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
  • Tissue specificity
    Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
  • Sequence similarities
    Belongs to the aldolase class II family. Adducin subfamily.
  • Domain
    Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADD A antibody
    • ADD1 antibody
    • ADDA antibody
    • ADDA_HUMAN antibody
    • Adducin 1 (alpha) antibody
    • Alpha adducin antibody
    • Alpha-adducin antibody
    • Erythrocyte adducin alpha subunit antibody
    • Erythrocyte adducin subunit alpha antibody
    • MGC3339 antibody
    • MGC44427 antibody
    • OTTHUMP00000151163 antibody
    • OTTHUMP00000151164 antibody
    • OTTHUMP00000151165 antibody
    • OTTHUMP00000218664 antibody
    • OTTHUMP00000218665 antibody
    see all


  • This image shows paraffin-embedded human brain tissue stained with ab51130 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue
  • All lanes : Anti-alpha Adducin antibody (ab51130)

    Lane 1 : Extracts from Hela cells treated
    with Forskolin (40nM, 30min) with no peptide
    Lane 2 : Extracts from Hela cells treated
    with Forskolin (40nM, 30min) with with immunising peptide

    Predicted band size: 81 kDa

  • ICC/IF image of ab51130 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51130, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab51130 used in Immunoprecipitation and Western Blot.
    Immunoprecipitation of NLN (EC, Thimet Oligopeptidase (thimet OP; EC, a-Adducin, and collapsin response mediator protein 2 (CRMP2) from photoradiolabeled P10 mouse forebrain membrane preparations. Immunoprecipitation of each protein was carried out from parallel nonspecific (NS) and total (Tot) photoradiolabeled groups.

    a, equal amounts of NS and total sample of each protein immunoprecipitate were used to count the radioactive signal. Bck, background radioactivity; ***, p < 0.001 versus all other groups; n = 3–4).

    b–e, representative Western blot confirmation of successful immunoprecipitation of each candidate protein from NS and total samples.


This product has been referenced in:
  • Han B  et al. Structural organization of the actin-spectrin-based membrane skeleton in dendrites and soma of neurons. Proc Natl Acad Sci U S A 114:E6678-E6685 (2017). Read more (PubMed: 28739933) »
  • Kugelmann D  et al. Adducin is involved in endothelial barrier stabilization. PLoS One 10:e0126213 (2015). Human . Read more (PubMed: 25978380) »

See all 6 Publications for this product

Customer reviews and Q&As

Thank you for your reply.

I have had the opportunity to view the exact immunogen sequence used for this product. While the exact information is considered proprietary, I can confirm that this immunogen is based on a region spanning aa 710 thr...

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Thank you for contacting us.

I have collected some data for you regarding these products as requested.
Regarding ab40760; this is predicted to cross-react with Beta-Adducin and Gamma-Adducin, based on sequence analysis. The immunogen sha...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab40760.

To check the status of the order pleas...

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I can send you a replacement vial of an anti-adducin antibody of your choice as we do not stock small sample sizes. So I can process your request, please include your PO number or Abcam order reference number associated with the purchase of ab51130, al...

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Thank you for your enquiry and for providing the attached western blot data.

Here are a few points to consider:

1. Since a small, non-phosphorylated peptide was used as the immunogen for ab51130, itwill not be reactive with total ad...

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