Anti-alpha Adducin antibody (ab51130)
Key features and details
- Rabbit polyclonal to alpha Adducin
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-alpha Adducin antibody
See all alpha Adducin primary antibodies -
Description
Rabbit polyclonal to alpha Adducin -
Host species
Rabbit -
Specificity
There are 2 isoforms of alpha adducin on the Uniprot protein database page for mouse. The antibody immunogen has high alignment to isoform 1: Isoform 1 80 kDa 100% Isoform 2 69 kDa 30% The aduccin protein is made up of 4 subunits, including the alpha beta and gamma subunits (according to the Uniprot page), Alignment of the immunogen with the mouse beta adducin: http://www.uniprot.org/uniprot/Q9QYB8 isoform 1 100% isoform 2 30% isoform 3 100% And with the mouse gamma adducin: http://www.uniprot.org/uniprot/Q9QYB5 isoform 1 92% Isoform 2 92% The antibody has not been specifically tested for detection of the other subunits, however according to the high alignments, the antibody is likely to detect several isoforms of these beta and gamma subunits. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
-
Immunogen
Synthesized non-phosphopeptide derived from human alpha Adducin around the phosphorylation site of serine 726 (T-P-SP-F-L).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
|
| WB |
1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
|
|
| ICC/IF | (1) |
Use a concentration of 1 - 5 µg/ml.
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
WB
1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa). |
|
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
Target
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Function
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. -
Tissue specificity
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit. -
Sequence similarities
Belongs to the aldolase class II family. Adducin subfamily. -
Domain
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region. -
Cellular localization
Cytoplasm > cytoskeleton. Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 118 Human
- Entrez Gene: 11518 Mouse
- Entrez Gene: 24170 Rat
- Omim: 102680 Human
- SwissProt: P35611 Human
- SwissProt: Q9QYC0 Mouse
- SwissProt: Q63028 Rat
- Unigene: 183706 Human
see all -
Alternative names
- ADD A antibody
- ADD1 antibody
- ADDA antibody
see all
Images
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Western blot - Anti-alpha Adducin antibody (ab51130)All lanes : Anti-alpha Adducin antibody (ab51130)
Lane 1 : Extracts from Hela cells treated
with Forskolin (40nM, 30min) with no peptide
Lane 2 : Extracts from Hela cells treated
with Forskolin (40nM, 30min) with with immunising peptide
Predicted band size: 81 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin antibody (ab51130)This image shows paraffin-embedded human brain tissue stained with ab51130 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue -
Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin antibody (ab51130)ICC/IF image of ab51130 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51130, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (10)
ab51130 has been referenced in 10 publications.
- Cabriel C et al. Combining 3D single molecule localization strategies for reproducible bioimaging. Nat Commun 10:1980 (2019). PubMed: 31040275
- Wang G et al. Structural plasticity of actin-spectrin membrane skeleton and functional role of actin and spectrin in axon degeneration. Elife 8:N/A (2019). PubMed: 31042147
- Gervasi MG et al. The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure. J Cell Sci 131:N/A (2018). PubMed: 29739876
- Hauser M et al. The Spectrin-Actin-Based Periodic Cytoskeleton as a Conserved Nanoscale Scaffold and Ruler of the Neural Stem Cell Lineage. Cell Rep 24:1512-1522 (2018). PubMed: 30089262
- Han B et al. Structural organization of the actin-spectrin-based membrane skeleton in dendrites and soma of neurons. Proc Natl Acad Sci U S A 114:E6678-E6685 (2017). PubMed: 28739933
- Kugelmann D et al. Adducin is involved in endothelial barrier stabilization. PLoS One 10:e0126213 (2015). Human . PubMed: 25978380
- Treweek JB et al. Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping. Nat Protoc 10:1860-1896 (2015). PubMed: 26492141
- Jones SL et al. Axon initial segment cytoskeleton comprises a multiprotein submembranous coat containing sparse actin filaments. J Cell Biol 205:67-81 (2014). PubMed: 24711503
- Zhong G et al. Developmental mechanism of the periodic membrane skeleton in axons. Elife 3:N/A (2014). Rat . PubMed: 25535840
- Wangler NJ et al. Identification of Membrane-bound Variant of Metalloendopeptidase Neurolysin (EC 3.4.24.16) as the Non-angiotensin Type 1 (Non-AT1), Non-AT2 Angiotensin Binding Site. J Biol Chem 287:114-22 (2012). WB, IP ; Mouse . PubMed: 22039052
