Overview

  • Product name
    Anti-alpha Adducin antibody [EP734Y]
    See all alpha Adducin primary antibodies
  • Description
    Rabbit monoclonal [EP734Y] to alpha Adducin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human alpha Adducin (C terminal). The exact sequence is proprietary.

  • Positive control
    • HeLa cell lysate, human lung (IHC-p).
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab40760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
IHC-P 1/250 - 1/500.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
    • Tissue specificity
      Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
    • Sequence similarities
      Belongs to the aldolase class II family. Adducin subfamily.
    • Domain
      Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADD A antibody
      • ADD1 antibody
      • ADDA antibody
      • ADDA_HUMAN antibody
      • Adducin 1 (alpha) antibody
      • Alpha adducin antibody
      • Alpha-adducin antibody
      • Erythrocyte adducin alpha subunit antibody
      • Erythrocyte adducin subunit alpha antibody
      • MGC3339 antibody
      • MGC44427 antibody
      • OTTHUMP00000151163 antibody
      • OTTHUMP00000151164 antibody
      • OTTHUMP00000151165 antibody
      • OTTHUMP00000218664 antibody
      • OTTHUMP00000218665 antibody
      see all

    Images

    • Anti-alpha Adducin antibody [EP734Y] (ab40760) at 1/50000 dilution + HeLa cell lysate at 10 µg

      Predicted band size: 120 kDa
      Observed band size: 120 kDa

    • ab40760 (1/250), staining human lung by immunohistochemistry, paraffin embedded tissue.
    • ab40760, (1/250) staining HeLa cells by immunofluorescence.

    References

    ab40760 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Answer

    Thank you for your reply.

    I have had the opportunity to view the exact immunogen sequence used for this product. While the exact information is considered proprietary, I can confirm that this immunogen is based on a region spanning aa 710 through to the C-terminus. I have not found why the post you found from 2006 mentions a different region.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



    Use our products? Submit an Abreview. Earn rewards!

    https://www.abcam.com/abreviews

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    Answer

    Thank you for contacting us.

    I have collected some data for you regarding these products as requested.
    Regarding ab40760; this is predicted to cross-react with Beta-Adducin and Gamma-Adducin, based on sequence analysis. The immunogen shares 100% and 94% sequence similarity with Beta-Adducin and Gamma-Adducin, respectively.

    Regarding ab51130; this product is also predicted to cross-react with the rat Beta-Adducin and rat Gamma-Adducin, also based on sequence analysis. The immunogen shares 96% and 91% sequence similarity with Beta-Adducin and Gamma-Adducin, respectively.

    These products are created from immunogens made of the C terminal region of alpha-adducin which shares high homology with the beta- and gamma- forms in mouse, human and rat. As such, we do guarantee them to work in those species in the applications listed on the specific datasheets. We try to include as much information as we have into these datasheets and do not know if these have been tested in other species or applications not listed.

    For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



    Use our products? Submit an Abreview. Earn rewards!

    https://www.abcam.com/abreviews

    Read More

    Answer

    The beta-adducin antibody is likely (although not guaranteed) to cross-react with alpha- or gamma- adducin. The immunogen shares 100% and 94% identity with alpha- and gamma-adducin, respectively.
    I hope this is helpful. Please contact me again if you have any further questions.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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