Key features and details
- Rabbit monoclonal [EP734Y] to alpha Adducin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-alpha Adducin antibody [EP734Y]
See all alpha Adducin primary antibodies
DescriptionRabbit monoclonal [EP734Y] to alpha Adducin
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human alpha Adducin (C terminal). The exact sequence is proprietary.
- HeLa cell lysate, human lung (IHC-p).
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab40760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
FunctionMembrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Tissue specificityExpressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
Sequence similaritiesBelongs to the aldolase class II family. Adducin subfamily.
DomainEach subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
Cellular localizationCytoplasm > cytoskeleton. Cell membrane.
- Information by UniProt
- ADD A antibody
- ADD1 antibody
- ADDA antibody
Anti-alpha Adducin antibody [EP734Y] (ab40760) at 1/50000 dilution + HeLa cell lysate at 10 µg
Predicted band size: 120 kDa
Observed band size: 120 kDa
ab40760 (1/250), staining human lung by immunohistochemistry, paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab40760, (1/250) staining HeLa cells by immunofluorescence.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab40760 has been referenced in 1 publication.
- Thauland TJ et al. The Actin-Capping Protein Alpha-Adducin Is Required for T-Cell Costimulation. Front Immunol 10:2706 (2019). PubMed: 31824498