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Inquiry: We recently purchased two alpha-adducin antibodies from you (ab51130 and ab40760). We now urgently need to know whether they cross-react with beta- or gamma- adducin. Could you please help us with this question (all concerning results on rat, ICC/IF but if you have info on other species/applications, that would be useful, too)?
Asked on Jun 19 2012
Thank you for contacting us.
I have collected some data for you regarding these products as requested.
Regarding ab40760; this is predicted to cross-react with Beta-Adducin and Gamma-Adducin, based on sequence analysis. The immunogen shares 100% and 94% sequence similarity with Beta-Adducin and Gamma-Adducin, respectively.
Regarding ab51130; this product is also predicted to cross-react with the rat Beta-Adducin and rat Gamma-Adducin, also based on sequence analysis. The immunogen shares 96% and 91% sequence similarity with Beta-Adducin and Gamma-Adducin, respectively.
These products are created from immunogens made of the C terminal region of alpha-adducin which shares high homology with the beta- and gamma- forms in mouse, human and rat. As such, we do guarantee them to work in those species in the applications listed on the specific datasheets. We try to include as much information as we have into these datasheets and do not know if these have been tested in other species or applications not listed.
For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Answered on Jun 19 2012