Anti-alpha Adducin antibody - N-terminal (ab151474)
- Datasheet
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Overview
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Product name
Anti-alpha Adducin antibody - N-terminal
See all alpha Adducin primary antibodies -
Description
Rabbit polyclonal to alpha Adducin - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to the N terminal amino acids 1-228 of Human alpha Adducin protein (P35611).
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Positive control
- WB: 293T, A431, H1299, HeLa, Molt-4, Raji and mouse liver lysates. IHC: SG xenograft.
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General notes
Keep as concentrated solution.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab151474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Detects a band of approximately 44 kDa (predicted molecular weight: 81 kDa). | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. -
Tissue specificity
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit. -
Sequence similarities
Belongs to the aldolase class II family. Adducin subfamily. -
Domain
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region. -
Cellular localization
Cytoplasm > cytoskeleton. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 118 Human
- Entrez Gene: 11518 Mouse
- Entrez Gene: 24170 Rat
- Entrez Gene: 555511 Zebrafish
- Omim: 102680 Human
- SwissProt: P35611 Human
- SwissProt: Q9QYC0 Mouse
- SwissProt: Q63028 Rat
see all -
Alternative names
- ADD A antibody
- ADD1 antibody
- ADDA antibody
see all
Images
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Western blot - Anti-alpha Adducin antibody - N-terminal (ab151474)Anti-alpha Adducin antibody - N-terminal (ab151474) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 81 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin antibody - N-terminal (ab151474)Immunohistochemical analysis of paraffin-embedded SG xenograft labeling alpha Adducin with ab151474 at 1/100. -
Western blot - Anti-alpha Adducin antibody - N-terminal (ab151474)Anti-alpha Adducin antibody - N-terminal (ab151474) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 81 kDa
7.5% SDS PAGE
References
ab151474 has not yet been referenced specifically in any publications.
