Overview

  • Product name

    Anti-alpha Adducin antibody - N-terminal
    See all alpha Adducin primary antibodies
  • Description

    Rabbit polyclonal to alpha Adducin - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to the N terminal amino acids 1-228 of Human alpha Adducin protein (P35611).

  • Positive control

    • WB: 293T, A431, H1299, HeLa, Molt-4, Raji and mouse liver lysates. IHC: SG xenograft.
  • General notes

    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab151474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Detects a band of approximately 44 kDa (predicted molecular weight: 81 kDa).
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
  • Tissue specificity

    Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
  • Sequence similarities

    Belongs to the aldolase class II family. Adducin subfamily.
  • Domain

    Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADD A antibody
    • ADD1 antibody
    • ADDA antibody
    • ADDA_HUMAN antibody
    • Adducin 1 (alpha) antibody
    • Alpha adducin antibody
    • Alpha-adducin antibody
    • Erythrocyte adducin alpha subunit antibody
    • Erythrocyte adducin subunit alpha antibody
    • MGC3339 antibody
    • MGC44427 antibody
    • OTTHUMP00000151163 antibody
    • OTTHUMP00000151164 antibody
    • OTTHUMP00000151165 antibody
    • OTTHUMP00000218664 antibody
    • OTTHUMP00000218665 antibody
    see all

Images

  • Anti-alpha Adducin antibody - N-terminal (ab151474) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 81 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded SG xenograft labeling alpha Adducin with ab151474 at 1/100.
  • Anti-alpha Adducin antibody - N-terminal (ab151474) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg

    Predicted band size: 81 kDa



    7.5% SDS PAGE

References

ab151474 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Permeabilization
Yes - citrate buffer
Specification
heart
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Jul 16 2018

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