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    alpha-adducin-antibody-n-terminal-ab151474.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin
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Anti-alpha Adducin antibody - N-terminal (ab151474)

  • Datasheet
  • SDS
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Western blot - Anti-alpha Adducin antibody - N-terminal (ab151474)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin antibody - N-terminal (ab151474)
  • Western blot - Anti-alpha Adducin antibody - N-terminal (ab151474)

Key features and details

  • Rabbit polyclonal to alpha Adducin - N-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-alpha Adducin antibody - N-terminal
    See all alpha Adducin primary antibodies
  • Description

    Rabbit polyclonal to alpha Adducin - N-terminal
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to the N terminal amino acids 1-228 of Human alpha Adducin protein (P35611).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: 293T, A431, H1299, HeLa, Molt-4, Raji and mouse liver lysates. IHC: SG xenograft.
  • General notes

    Keep as concentrated solution.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKC
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKA
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calmodulin Pathway

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Mouse liver tissue lysate - total protein (ab29301)
    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • A-431 whole cell lysate (ab30132)
    • A-431 whole cell lysate (ab7909)
    • MOLT-4 whole cell lysate (ab7912)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Human
All applications
Rat
Chicken
Cow
Xenopus laevis
Zebrafish
Xenopus tropicalis
Application Abreviews Notes
WB
1/500 - 1/3000. Detects a band of approximately 44 kDa (predicted molecular weight: 81 kDa).
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/500 - 1/3000. Detects a band of approximately 44 kDa (predicted molecular weight: 81 kDa).
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
  • Tissue specificity

    Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
  • Sequence similarities

    Belongs to the aldolase class II family. Adducin subfamily.
  • Domain

    Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell membrane.
  • Target information above from: UniProt accession P35611 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 118 Human
    • Entrez Gene: 11518 Mouse
    • Entrez Gene: 24170 Rat
    • Entrez Gene: 555511 Zebrafish
    • Omim: 102680 Human
    • SwissProt: P35611 Human
    • SwissProt: Q9QYC0 Mouse
    • SwissProt: Q63028 Rat
    • Unigene: 183706 Human
    • Unigene: 289106 Mouse
    • Unigene: 5788 Rat
    see all
  • Alternative names

    • ADD A antibody
    • ADD1 antibody
    • ADDA antibody
    • ADDA_HUMAN antibody
    • Adducin 1 (alpha) antibody
    • Alpha adducin antibody
    • Alpha-adducin antibody
    • Erythrocyte adducin alpha subunit antibody
    • Erythrocyte adducin subunit alpha antibody
    • MGC3339 antibody
    • MGC44427 antibody
    • OTTHUMP00000151163 antibody
    • OTTHUMP00000151164 antibody
    • OTTHUMP00000151165 antibody
    • OTTHUMP00000218664 antibody
    • OTTHUMP00000218665 antibody
    see all

Images

  • Western blot - Anti-alpha Adducin antibody - N-terminal (ab151474)
    Western blot - Anti-alpha Adducin antibody - N-terminal (ab151474)
    Anti-alpha Adducin antibody - N-terminal (ab151474) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 81 kDa



    10% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin antibody - N-terminal (ab151474)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin antibody - N-terminal (ab151474)
    Immunohistochemical analysis of paraffin-embedded SG xenograft labeling alpha Adducin with ab151474 at 1/100.
  • Western blot - Anti-alpha Adducin antibody - N-terminal (ab151474)
    Western blot - Anti-alpha Adducin antibody - N-terminal (ab151474)
    Anti-alpha Adducin antibody - N-terminal (ab151474) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg

    Predicted band size: 81 kDa



    7.5% SDS PAGE

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab151474? Please let us know so that we can cite the reference in this datasheet.

    ab151474 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Immunohistochemistry (Frozen sections) abreview for Anti-alpha Adducin antibody - N-terminal

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (heart)
    Permeabilization
    Yes - citrate buffer
    Specification
    heart
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 16 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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