Anti-alpha Adducin antibody - N-terminal (ab151474)
Key features and details
- Rabbit polyclonal to alpha Adducin - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-alpha Adducin antibody - N-terminal
See all alpha Adducin primary antibodies -
Description
Rabbit polyclonal to alpha Adducin - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Recombinant fragment corresponding to the N terminal amino acids 1-228 of Human alpha Adducin protein (P35611).
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Positive control
- WB: 293T, A431, H1299, HeLa, Molt-4, Raji and mouse liver lysates. IHC: SG xenograft.
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General notes
Keep as concentrated solution.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Mouse
Human
|
| All applications |
Rat
Chicken
Cow
Xenopus laevis
Zebrafish
Xenopus tropicalis
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Detects a band of approximately 44 kDa (predicted molecular weight: 81 kDa).
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Detects a band of approximately 44 kDa (predicted molecular weight: 81 kDa). |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. -
Tissue specificity
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit. -
Sequence similarities
Belongs to the aldolase class II family. Adducin subfamily. -
Domain
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region. -
Cellular localization
Cytoplasm > cytoskeleton. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 118 Human
- Entrez Gene: 11518 Mouse
- Entrez Gene: 24170 Rat
- Entrez Gene: 555511 Zebrafish
- Omim: 102680 Human
- SwissProt: P35611 Human
- SwissProt: Q9QYC0 Mouse
- SwissProt: Q63028 Rat
see all -
Alternative names
- ADD A antibody
- ADD1 antibody
- ADDA antibody
see all
Images
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Western blot - Anti-alpha Adducin antibody - N-terminal (ab151474)Anti-alpha Adducin antibody - N-terminal (ab151474) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 81 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin antibody - N-terminal (ab151474)Immunohistochemical analysis of paraffin-embedded SG xenograft labeling alpha Adducin with ab151474 at 1/100. -
Western blot - Anti-alpha Adducin antibody - N-terminal (ab151474)Anti-alpha Adducin antibody - N-terminal (ab151474) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 81 kDa
7.5% SDS PAGE
Protocols
References (0)
ab151474 has not yet been referenced specifically in any publications.
