Product nameAnti-alpha Adducin (phospho S59) antibody [EP2175AY]
See all alpha Adducin primary antibodies
DescriptionRabbit monoclonal [EP2175AY] to alpha Adducin (phospho S59)
Specificityab76251 only detects alpha Adducin when phosphorylated at Serine 59.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Human
Synthetic phospho-peptide corresponding to residues surrounding Serine59 of human alpha Adducin
- HeLa cell lysates, untreated, or treated with calyculin A
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/5000. Predicted molecular weight: 81 kDa.
Is unsuitable for Flow Cyt, ICC and IHC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMembrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Tissue specificityExpressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
Sequence similaritiesBelongs to the aldolase class II family. Adducin subfamily.
DomainEach subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
Cellular localizationCytoplasm > cytoskeleton. Cell membrane.
- Information by UniProt
- ADD A antibody
- ADD1 antibody
- ADDA antibody
All lanes : Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251) at 1/10000 dilution
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate, treated with calyculin A
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP labelled IgG at 1/1000 dilution
Predicted band size: 81 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
ab76251 has not yet been referenced specifically in any publications.