Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2175AY] to alpha Adducin (phospho S59)
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-alpha Adducin (phospho S59) antibody [EP2175AY]
See all alpha Adducin primary antibodies
DescriptionRabbit monoclonal [EP2175AY] to alpha Adducin (phospho S59)
Specificityab76251 only detects alpha Adducin when phosphorylated at Serine 59.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Human
Synthetic phospho-peptide corresponding to residues surrounding Serine59 of human alpha Adducin
- HeLa cell lysates, untreated, or treated with calyculin A
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/5000. Predicted molecular weight: 81 kDa.
Is unsuitable for Flow Cyt, ICC and IHC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMembrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Tissue specificityExpressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
Sequence similaritiesBelongs to the aldolase class II family. Adducin subfamily.
DomainEach subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
Cellular localizationCytoplasm > cytoskeleton. Cell membrane.
- Information by UniProt
- ADD A antibody
- ADD1 antibody
- ADDA antibody
All lanes : Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251) at 1/10000 dilution
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate, treated with calyculin A
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP labelled IgG at 1/1000 dilution
Predicted band size: 81 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
ab76251 has not yet been referenced specifically in any publications.