Key features and details
- Rabbit polyclonal to alpha Adducin (phospho S726)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-alpha Adducin (phospho S726) antibody
See all alpha Adducin primary antibodies
DescriptionRabbit polyclonal to alpha Adducin (phospho S726)
Specificityab53093 detects endogenous levels of alpha Adducin only when phosphorylated at serine 726.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic phosphopeptide derived from human alpha Adducin around the phosphorylation site of serine 726 (TPSPFL).
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab53093 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab53093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionMembrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Tissue specificityExpressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
Sequence similaritiesBelongs to the aldolase class II family. Adducin subfamily.
DomainEach subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
Cellular localizationCytoplasm > cytoskeleton. Cell membrane.
- Information by UniProt
- ADD A antibody
- ADD1 antibody
- ADDA antibody
All lanes : Anti-alpha Adducin (phospho S726) antibody (ab53093) at 1/500 dilution
Lane 1 : HeLa cell extract treated with Forskolin (40nM, 30 mins) with phosphopeptide
Lane 2 : HeLa cell extract treated with Forskolin (40nM, 30 mins)
Predicted band size: 81 kDa
Observed band size: 81 kDa
labelled with ab53093, at 1/50 dilution, staining alpha Adducin in paraffin embedded human brain tissue sections by Immunohistochemistry. Tissues had either been treated with (right image) or without (left image) the phosphopeptide.
ab53093 has been referenced in 1 publication.
- Tura A et al. Efficacy of Rho-kinase inhibition in promoting cell survival and reducing reactive gliosis in the rodent retina. Invest Ophthalmol Vis Sci 50:452-61 (2009). PubMed: 18757509