Anti-alpha Adducin (phospho S726) antibody (ab53093)


  • Product name
    Anti-alpha Adducin (phospho S726) antibody
    See all alpha Adducin primary antibodies
  • Description
    Rabbit polyclonal to alpha Adducin (phospho S726)
  • Host species
  • Specificity
    ab53093 detects endogenous levels of alpha Adducin only when phosphorylated at serine 726.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human alpha Adducin around the phosphorylation site of serine 726 (TPSPFL).

  • Positive control
    • WB: Human brain tissue IHC-P: HeLa cell extract treated with Forskolin This antibody gave a positive result when used in the following formaldehyde fixed cell lines:SKNSH.



Our Abpromise guarantee covers the use of ab53093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
ICC/IF 1/100 - 1/500.
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.


  • Function
    Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
  • Tissue specificity
    Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
  • Sequence similarities
    Belongs to the aldolase class II family. Adducin subfamily.
  • Domain
    Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADD A antibody
    • ADD1 antibody
    • ADDA antibody
    • ADDA_HUMAN antibody
    • Adducin 1 (alpha) antibody
    • Alpha adducin antibody
    • Alpha-adducin antibody
    • Erythrocyte adducin alpha subunit antibody
    • Erythrocyte adducin subunit alpha antibody
    • MGC3339 antibody
    • MGC44427 antibody
    • OTTHUMP00000151163 antibody
    • OTTHUMP00000151164 antibody
    • OTTHUMP00000151165 antibody
    • OTTHUMP00000218664 antibody
    • OTTHUMP00000218665 antibody
    see all


  • labelled with ab53093, at 1/50 dilution, staining alpha Adducin in paraffin embedded human brain tissue sections by Immunohistochemistry. Tissues had either been treated with (right image) or without (left image) the phosphopeptide.
  • All lanes : Anti-alpha Adducin (phospho S726) antibody (ab53093) at 1/500 dilution

    Lane 1 : HeLa cell extract treated with Forskolin (40nM, 30 mins) with phosphopeptide
    Lane 2 : HeLa cell extract treated with Forskolin (40nM, 30 mins)

    Predicted band size: 81 kDa
    Observed band size: 81 kDa

  • ab53093 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab53093 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Tura A  et al. Efficacy of Rho-kinase inhibition in promoting cell survival and reducing reactive gliosis in the rodent retina. Invest Ophthalmol Vis Sci 50:452-61 (2009). Read more (PubMed: 18757509) »

See 1 Publication for this product

Customer reviews and Q&As

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab40760.

To check the status of the order pleas...

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I can send you a replacement vial of an anti-adducin antibody of your choice as we do not stock small sample sizes. So I can process your request, please include your PO number or Abcam order reference number associated with the purchase of ab51130, al...

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Thank you for your enquiry and for providing the attached western blot data.

Here are a few points to consider:

1. Since a small, non-phosphorylated peptide was used as the immunogen for ab51130, itwill not be reactive with total ad...

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