Anti-alpha Adducin (phospho S726) antibody (ab53093)
Key features and details
- Rabbit polyclonal to alpha Adducin (phospho S726)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-alpha Adducin (phospho S726) antibody
See all alpha Adducin primary antibodies -
Description
Rabbit polyclonal to alpha Adducin (phospho S726) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human alpha Adducin aa 650-750 (phospho S726). Swiss-Prot #: P35611/P35612
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Positive control
- WB: HeLa cells. IHC-P: Human Brain and Human Breast carcinoma tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab53093 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa). |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/100 - 1/500. |
Target
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Function
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. -
Tissue specificity
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit. -
Sequence similarities
Belongs to the aldolase class II family. Adducin subfamily. -
Domain
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region. -
Cellular localization
Cytoplasm > cytoskeleton. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 118 Human
- Omim: 102680 Human
- SwissProt: P35611 Human
- Unigene: 183706 Human
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Alternative names
- ADD A antibody
- ADD1 antibody
- ADDA antibody
see all
Images
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All lanes : Anti-alpha Adducin (phospho S726) antibody (ab53093) at 1/500 dilution
Lane 1 : HeLa cells treated with Forskolin (40nM 30 mins)
Lane 2 : HeLa cells treated with Forskolin (40nM 30 mins) and blocked with the phospho peptide.
Predicted band size: 81 kDa -
labelled with ab53093, at 1/50 dilution, staining alpha Adducin in paraffin embedded human brain tissue sections by Immunohistochemistry. Tissues had either been treated with (right image) or without (left image) the phosphopeptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ab53093 at 1/50. The picture on the right is blocked with the phospo peptide.
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Immunocytochemistry analysis of HeLa cells, using ab53093 at 1/100 dilution. The picture on the right is blocked with the phospho peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab53093 has been referenced in 2 publications.
- Ferrer I et al. Dysregulated protein phosphorylation: A determining condition in the continuum of brain aging and Alzheimer's disease. Brain Pathol 31:e12996 (2021). PubMed: 34218486
- Tura A et al. Efficacy of Rho-kinase inhibition in promoting cell survival and reducing reactive gliosis in the rodent retina. Invest Ophthalmol Vis Sci 50:452-61 (2009). PubMed: 18757509